Assessing the contribution of the neutral blocks in DNA/Block-copolymer polyplexes : poly(acrylamide) vs. poly(ethylene oxide)
Renata Mellogiona, Letícia Vitorazi, Watson Loh
ARTIGO
Inglês
Agradecimentos: The authors would like to acknowledge FAPESP for the financial support through thematic projects 2015/25406-5 and 2021/12071-6 and through a postdoctoral grant to RMG (10/00528-7). We thank INCT-Catálise, Marcos Vinicius Aquino Queirós, Guilherme C. Campese, Professor José Adilson de...
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Agradecimentos: The authors would like to acknowledge FAPESP for the financial support through thematic projects 2015/25406-5 and 2021/12071-6 and through a postdoctoral grant to RMG (10/00528-7). We thank INCT-Catálise, Marcos Vinicius Aquino Queirós, Guilherme C. Campese, Professor José Adilson de Castro, and Professor Karin Schillén for helpful discussions. We would like to dedicate this article to Professor Bjorn Lindman on the occasion of his 80th birthday. We acknowledge him as a role model as a scientist, for his many important contributions to Colloid Science, and for his interest in and support for our research activities. This research was funded by FAPESP through thematic projects 2015/25406-5 and 2021/12071-6 and through a postdoctoral grant to RMG (10/00528-7)
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Abstract: The interaction of DNA with different block copolymers, namely poly (trimethylammonium chloride methacryloyoxy)ethyl)-block-poly(acrylamide), i.e., (PTEA)-b-(PAm), and poly (trimethylammonium chloride methacryloyoxy)ethyl)-block-poly(ethylene oxide), i.e., (PTEA)-b-(PEO), was studied. The...
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Abstract: The interaction of DNA with different block copolymers, namely poly (trimethylammonium chloride methacryloyoxy)ethyl)-block-poly(acrylamide), i.e., (PTEA)-b-(PAm), and poly (trimethylammonium chloride methacryloyoxy)ethyl)-block-poly(ethylene oxide), i.e., (PTEA)-b-(PEO), was studied. The nature of the cationic block was maintained fixed (PTEA), whereas the neutral blocks contained varying amounts of acrylamide or (ethylene oxide) units. According to results from isothermal titration microcalorimetry measurements, the copolymers interaction with DNA is endothermic with an enthalpy around 4.0 kJ mol-1 of charges for (PTEA)-b-(PAm) and 5.5 kJ mol-1 of charges for (PTEA)-b-(PEO). The hydrodynamic diameters of (PTEA)-b-(PEO)/DNA and (PTEA)-b-(PAm)/DNA polyplexes prepared by titration were around 200 nm at charge ratio (Z+/-) < 1. At Z+/- close and above 1, the (PTEA)50-b-(PAm)50/DNA and (PTEA)50-b-(PAm)200/DNA polyplexes precipitated. Interestingly, (PTEA)50-b-(PAm)1000/DNA polyplexes remained with a size of around 300 nm even after charge neutralization, probably due to the size of the neutral block. Conversely, for (PTEA)96-b-(PEO)100/DNA polyplexes, the size distribution was broad, indicating a more heterogeneous system. Polyplexes were also prepared by direct mixture at Z+/- of 2.0, and they displayed diameters around 120-150 nm, remaining stable for more than 10 days. Direct and reverse titration experiments showed that the order of addition affects both the size and charge of the resulting polyplexes
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2015/25406-5; 2021/12071-6
Aberto
DOI: https://doi.org/10.3390/molecules28010398
Texto completo: https://www.mdpi.com/1420-3049/28/1/398
Assessing the contribution of the neutral blocks in DNA/Block-copolymer polyplexes : poly(acrylamide) vs. poly(ethylene oxide)
Renata Mellogiona, Letícia Vitorazi, Watson Loh
Assessing the contribution of the neutral blocks in DNA/Block-copolymer polyplexes : poly(acrylamide) vs. poly(ethylene oxide)
Renata Mellogiona, Letícia Vitorazi, Watson Loh
Fontes
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Molecules: a journal of synthetic organic and natural product chemistry v. 28, n. 398, p. 2-16, Jan. 2023 |