Agradecimentos: ACKNOWLEDGEMENTS This study was supported by the following Brazilian institutions: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Grant No. 2020/07741-0; 2019/07838-6), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Finance Code 001 and...

Agradecimentos: ACKNOWLEDGEMENTS This study was supported by the following Brazilian institutions: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Grant No. 2020/07741-0; 2019/07838-6), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Finance Code 001 and CAPES/PrInt, fellowship awarded to NRS) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Abstract: The modern cultivars of sugarcane (Saccharum spp.) are highly polyploid and accumulate aneuploidies due to their history of domestication, genetic improvement and interspecific hybrid origin involving the domesticated sweet species Saccharum officinarum (‘noble cane’) and the wild...

Abstract: The modern cultivars of sugarcane (Saccharum spp.) are highly polyploid and accumulate aneuploidies due to their history of domestication, genetic improvement and interspecific hybrid origin involving the domesticated sweet species Saccharum officinarum (‘noble cane’) and the wild Saccharum spontaneum, both with an evolutionary history of polyploidy. The first hybrids were backcrossed with S. officinarum, and selection from progenies in subsequent generations established the genetic basis of modern cultivars. Saccharum genome complexity has inspired several molecular studies that have elucidated aspects of sugarcane genome constitution, architecture and cytogenetics. Herein, we conducted a comparative analysis of the meiotic behaviour of representatives of the parentals S. officinarum and S. spontaneum, and the commercial variety, SP80-3280. S. officinarum, an octoploid species, exhibited regular meiotic behaviour. In contrast, S. spontaneum and SP80-3280 exhibited several abnormalities from metaphase I to the end of division. We reported and typified, for the first time, the occurrence of peri- and paracentric inversions. Using in-situ hybridisation techniques, we were able to determine how pairing association occurred at diakinesis, the origin of lagging chromosomes and, in particular, the mitotic chromosome composition of SP80-3280. Interestingly, S. spontaneum and recombinant chromosomes showed the most marked tendency to produce laggards in both divisions. Future attempts to advance knowledge on sugarcane genetics and genomics should take meiotic chromosome behaviour information into account

FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP

2019/07838-6; 2020/07741-0

COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES

001

CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ

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