Yeast population dynamics in brazilian bioethanol production
Artur Rego-Costa, I Ting Huang, Michael M Desai, Andreas K Gombert
ARTIGO
Inglês
Agradecimentos: We thank the various members of Site A and Site B who provided extensive support and expertise along the way, specially Thaís Granço and Luciano Zamberlan. We thank the Bauer Core facility at Harvard for assistance with sequencing
Abstract: The large-scale and nonaseptic fermentation of sugarcane feedstocks into fuel ethanol in biorefineries represents a unique ecological niche, in which the yeast Saccharomyces cerevisiae is the predominant organism. Several factors, such as sugarcane variety, process design, and operating...
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Abstract: The large-scale and nonaseptic fermentation of sugarcane feedstocks into fuel ethanol in biorefineries represents a unique ecological niche, in which the yeast Saccharomyces cerevisiae is the predominant organism. Several factors, such as sugarcane variety, process design, and operating and weather conditions, make each of the ~400 industrial units currently operating in Brazil a unique ecosystem. Here, we track yeast population dynamics in 2 different biorefineries through 2 production seasons (April to November of 2018 and 2019), using a novel statistical framework on a combination of metagenomic and clonal sequencing data. We find that variation from season to season in 1 biorefinery is small compared to the differences between the 2 units. In 1 biorefinery, all lineages present during the entire production period derive from 1 of the starter strains, while in the other, invading lineages took over the population and displaced the starter strain. However, despite the presence of invading lineages and the nonaseptic nature of the process, all yeast clones we isolated are phylogenetically related to other previously sequenced bioethanol yeast strains, indicating a common origin from this industrial niche. Despite the substantial changes observed in yeast populations through time in each biorefinery, key process indicators remained quite stable through both production seasons, suggesting that the process is robust to the details of these population dynamics
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2018/04962-5
Aberto
Yeast population dynamics in brazilian bioethanol production
Artur Rego-Costa, I Ting Huang, Michael M Desai, Andreas K Gombert
Yeast population dynamics in brazilian bioethanol production
Artur Rego-Costa, I Ting Huang, Michael M Desai, Andreas K Gombert
Fontes
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G3: genes, genomes, genetics (Bethesda) v.13, n.7, p.1-12, 2023 |