Podoplanin and clec-2 levels in patients with covid-19
Ivanio Teixeira Borba Junior, Franciele Lima, Davi Sidarta Vitoria Rodrigues Oliveira, Carla Roberta Peachazepi Moraes, Joyce M. Annichino-Bizzacchi, Bruna Bombassaro, André C. Palma, Fabio Trindade Maranhao Costa, Maria Luiza Moretti, Eli Mansour, Licio Augusto Velloso, Fernanda Andrade Orsi, Erich...
Ivanio Teixeira Borba Junior, Franciele Lima, Davi Sidarta Vitoria Rodrigues Oliveira, Carla Roberta Peachazepi Moraes, Joyce M. Annichino-Bizzacchi, Bruna Bombassaro, André C. Palma, Fabio Trindade Maranhao Costa, Maria Luiza Moretti, Eli Mansour, Licio Augusto Velloso, Fernanda Andrade Orsi, Erich Vinicius de Paula
ARTIGO
Inglês
Agradecimentos: This study was funded by the Sao Paulo Research Foundation grants 2016/14172-6, 2020/05985-9, and 2020/05369-6; FAEPEX-Unicamp grant 2404/2020; and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior–Brasil, finance code 001.
Abstract: Podoplanin (PDPN gene) and CLEC-2 are involved in inflammatory hemostasis and have also been related with the pathogenesis of thrombosis. Emerging evidence also suggest that podoplanin can exert protective effects in sepsis and in acute lung injury. In lungs, podoplanin is co-expressed...
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Abstract: Podoplanin (PDPN gene) and CLEC-2 are involved in inflammatory hemostasis and have also been related with the pathogenesis of thrombosis. Emerging evidence also suggest that podoplanin can exert protective effects in sepsis and in acute lung injury. In lungs, podoplanin is co-expressed with ACE2, which is the main entry receptor for SARS-CoV-2. To explore the role of podoplanin and CLEC-2 in COVID-19. Circulating levels of podoplanin and CLEC-2 were measured in 30 consecutive COVID-19 patients admitted due to hypoxia, and in 30 age- and sex-matched healthy individuals. Podoplanin expression in lungs from patients who died of COVID-19 was obtained from two independent public databases of single-cell RNAseq from which data from control lungs were also available. Circulating podoplanin levels were lower in COVID-19, while no difference was observed in CLEC-2 levels. Podoplanin levels were significantly inversely correlated with markers of coagulation, fibrinolysis and innate immunity. scRNAseq data confirmed that PDPN is co-expressed with ACE2 in pneumocytes, and showed that PDPN expression is lower in this cell compartment in lungs from patients with COVID-19. Circulating levels of podoplanin are lower in COVID-19, and the magnitude of this reduction is correlated with hemostasis activation. We also demonstrate the downregulation of PDPN at the transcription level in pneumocytes. Together, our exploratory study questions whether an acquired podoplanin deficiency could be involved in the pathogenesis of acute lung injury in COVID-19, and warrant additional studies to confirm and refine these findings
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2016/14172-6; 2020/05369-6; 2020/05985-9
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
001
Aberto
Bombassaro, Bruna, 1989-
Autor
Mansur, Eli
Autor
Podoplanin and clec-2 levels in patients with covid-19
Ivanio Teixeira Borba Junior, Franciele Lima, Davi Sidarta Vitoria Rodrigues Oliveira, Carla Roberta Peachazepi Moraes, Joyce M. Annichino-Bizzacchi, Bruna Bombassaro, André C. Palma, Fabio Trindade Maranhao Costa, Maria Luiza Moretti, Eli Mansour, Licio Augusto Velloso, Fernanda Andrade Orsi, Erich...
Ivanio Teixeira Borba Junior, Franciele Lima, Davi Sidarta Vitoria Rodrigues Oliveira, Carla Roberta Peachazepi Moraes, Joyce M. Annichino-Bizzacchi, Bruna Bombassaro, André C. Palma, Fabio Trindade Maranhao Costa, Maria Luiza Moretti, Eli Mansour, Licio Augusto Velloso, Fernanda Andrade Orsi, Erich Vinicius de Paula
Podoplanin and clec-2 levels in patients with covid-19
Ivanio Teixeira Borba Junior, Franciele Lima, Davi Sidarta Vitoria Rodrigues Oliveira, Carla Roberta Peachazepi Moraes, Joyce M. Annichino-Bizzacchi, Bruna Bombassaro, André C. Palma, Fabio Trindade Maranhao Costa, Maria Luiza Moretti, Eli Mansour, Licio Augusto Velloso, Fernanda Andrade Orsi, Erich...
Ivanio Teixeira Borba Junior, Franciele Lima, Davi Sidarta Vitoria Rodrigues Oliveira, Carla Roberta Peachazepi Moraes, Joyce M. Annichino-Bizzacchi, Bruna Bombassaro, André C. Palma, Fabio Trindade Maranhao Costa, Maria Luiza Moretti, Eli Mansour, Licio Augusto Velloso, Fernanda Andrade Orsi, Erich Vinicius de Paula
Fontes
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Research and practice in thrombosis and haemostasis v. 7, n. 5, n. art. 100282, July 2023 |