Lipidome signatures in early bovine embryo development
Mateus J. Sudano, Tatiana D. S. Rascado, Alessandra Tata, Katia R. A. Belaz, Vanessa G. Santos, Roniele S. Valente, Fernando S. Mesquita, Christina R. Ferreira, João P. Araújo, Marcos N. Eberlin, Fernanda D. C. Landim-Alvarenga
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Agradecimentos: This research was supported by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq, Brasil (grant 446647/2014-4; fellowships 162598/2013-0 and 167908/2013-8) and Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP (fellowships 2011/06191-7 and 2012/07206-0)....
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Agradecimentos: This research was supported by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq, Brasil (grant 446647/2014-4; fellowships 162598/2013-0 and 167908/2013-8) and Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP (fellowships 2011/06191-7 and 2012/07206-0). Author contributions: MJS and FDCLA conceived and designed the experiments; TDSR, AT, KRAB, and VGS performed the experiments; MJS and RSV analyzed the data; FDCLA, MJS, JPA, and MNE contributed reagents/materials/analysis tool; and MJS, FSM, and CRF wrote the article
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Abstract: Mammalian preimplantation embryonic development is a complex, conserved, and well orchestrated process involving dynamic molecular and structural changes. Understanding membrane lipid profile fluctuation during this crucial period is fundamental to address mechanisms governing...
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Abstract: Mammalian preimplantation embryonic development is a complex, conserved, and well orchestrated process involving dynamic molecular and structural changes. Understanding membrane lipid profile fluctuation during this crucial period is fundamental to address mechanisms governing embryogenesis. Therefore, the aim of the present work was to perform a comprehensive assessment of stage-specific lipid profiles during early bovine embryonic development and associate with the mRNA abundance of lipid metabolism related genes (ACSL3, ELOVL5, and ELOVL6) and with the amount of cytoplasmic lipid droplets. Immature oocytes were recovered from slaughterhouse-derived ovaries, two-cell embryos, and eight- to 16-cell embryos, morula, and blastocysts that were in vitro produced under different environmental conditions. Lipid droplets content and mRNA transcript levels for ACSL3, ELOVL5, and ELOVL6, monitored by lipid staining and quantitative polymerase chain reaction, respectively, increased at morula followed by a decrease at blastocyst stage. Relative mRNA abundance changes of ACSL3 were closely related to cytoplasmic lipid droplet accumulation. Characteristic dynamic changes of phospholipid profiles were observed during early embryo development and related to unsaturation level, acyl chain length, and class composition. ELOVL5 and ELOVL6 mRNA levels were suggestive of over expression of membrane phospholipids containing elongated fatty acids with 16, 18, and 20 carbons. In addition, putative biomarkers of key events of embryogenesis, embryo lipid accumulation, and elongation were identified. This study provides a comprehensive description of stage-specific lipidome signatures and proposes a mechanism to explain its potential relationship with the fluctuation of both cytoplasmic lipid droplets content and mRNA levels of lipid metabolism-related genes during early bovine embryo development
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CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
446647/2014-4; 162598/2013-0; 167908/2013-8
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2011/06191-7; 2012/07206-0
Aberto
Lipidome signatures in early bovine embryo development
Mateus J. Sudano, Tatiana D. S. Rascado, Alessandra Tata, Katia R. A. Belaz, Vanessa G. Santos, Roniele S. Valente, Fernando S. Mesquita, Christina R. Ferreira, João P. Araújo, Marcos N. Eberlin, Fernanda D. C. Landim-Alvarenga
Lipidome signatures in early bovine embryo development
Mateus J. Sudano, Tatiana D. S. Rascado, Alessandra Tata, Katia R. A. Belaz, Vanessa G. Santos, Roniele S. Valente, Fernando S. Mesquita, Christina R. Ferreira, João P. Araújo, Marcos N. Eberlin, Fernanda D. C. Landim-Alvarenga
Fontes
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Theriogenology Vol. 86, n. 2 (July, 2016), p. 472-484 |