Silver nanoparticles (AgNPs) have been extensively studied because of their anti-microbial potential. Here, we evaluated the effect of biologically synthesized silver nanoparticles (AgNP bio ) alone and in combination with fluconazole (FLC) against planktonic cells and biofilms of FLC-resistant...

Silver nanoparticles (AgNPs) have been extensively studied because of their anti-microbial potential. Here, we evaluated the effect of biologically synthesized silver nanoparticles (AgNP bio ) alone and in combination with fluconazole (FLC) against planktonic cells and biofilms of FLC-resistant Candida albicans . AgNP bio exhibited a fungicidal effect, with a minimal inhibitory concentration (MIC) and fungicidal concentration ranging from 2.17 to 4.35 μg/ml. The combination of AgNP bio and FLC reduced the MIC of FLC around 16 to 64 times against planktonic cells of all C. albicans . There was no significant inhibitory effect of AgNP bio on biofilm cells. However, FLC combined with AgNP bio caused a significant dose-dependent decrease in the viability of both initial and mature biofilm. All concentrations of AgNP bio , alone or in combination with FLC, were not cytotoxic to mammalian cells. The results highlight the effectiveness of the combination of AgNP bio with FLC against FLC-resistant C. albicans .

COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES

402728/2013-0

CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ

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