Crystal structure and regulation of the citrus Pol III repressor MAF1 by auxin and phosphorylation
Adriana Santos Soprano, Priscila Oliveira de Giuseppe, Hugo Massayoshi Shimo, Tatiani Brenelli Lima, Fernanda Aparecida Heleno Batista, Germanna Lima Righetto, Jose Geraldo de Carvalho Pereira, Daniela Campos Granato, Andrey Fabricio Ziem Nascimento, Fabio Cesar Gozzo, Paulo Sergio Lopes de...
Adriana Santos Soprano, Priscila Oliveira de Giuseppe, Hugo Massayoshi Shimo, Tatiani Brenelli Lima, Fernanda Aparecida Heleno Batista, Germanna Lima Righetto, Jose Geraldo de Carvalho Pereira, Daniela Campos Granato, Andrey Fabricio Ziem Nascimento, Fabio Cesar Gozzo, Paulo Sergio Lopes de Oliveira, Ana Carolina Migliorini Figueira, Juliana Helena Costa Smetana, Adriana Franco Paes Leme, Mario Tyago Murakami, Celso Eduardo Benedetti
ARTIGO
Inglês
Agradecimentos: This work was supported by São Paulo Research Foundation (FAPESP grant 2011/20468-1). C.E.B. and A.F.Z.N. received a fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). We thank Jaqueline Silva, Gustavo Mercaldi, and Jackeline Zanella for helping...
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Agradecimentos: This work was supported by São Paulo Research Foundation (FAPESP grant 2011/20468-1). C.E.B. and A.F.Z.N. received a fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). We thank Jaqueline Silva, Gustavo Mercaldi, and Jackeline Zanella for helping with protein expression and purification. We also thank the technical support team of the mass spectrometry laboratory of LNBio, Romenia Domingues and Bianca Pauletti, and the Synchrotron Light Laboratory (LNLS) MX2 X-ray beamline at the Brazilian Nacional Center for Energy and Material Science (CNPEM), as well as the XALOC beamline at ALBA Synchrotron
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MAF1 is the main RNA polymerase (Pol) III repressor that controls cell growth in eukaryotes. The Citrus ortholog, CsMAF1, was shown to restrict cell growth in citrus canker disease but its role in plant development and disease is still unclear. We solved the crystal structure of the globular core of...
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MAF1 is the main RNA polymerase (Pol) III repressor that controls cell growth in eukaryotes. The Citrus ortholog, CsMAF1, was shown to restrict cell growth in citrus canker disease but its role in plant development and disease is still unclear. We solved the crystal structure of the globular core of CsMAF1, which reveals additional structural elements compared with the previously available structure of hMAF1, and explored the dynamics of its flexible regions not present in the structure. CsMAF1 accumulated in the nucleolus upon leaf excision, and this translocation was inhibited by auxin and by mutation of the PKA phosphorylation site, S45, to aspartate. Additionally, mTOR phosphorylated recombinant CsMAF1 and the mTOR inhibitor AZD8055 blocked canker formation in normal but not CsMAF1-silenced plants. These results indicate that the role of TOR on cell growth induced by Xanthomonas citri depends on CsMAF1 and that auxin controls CsMAF1 interaction with Pol III in citrus
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2011/20468-1
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
Fechado
Crystal structure and regulation of the citrus Pol III repressor MAF1 by auxin and phosphorylation
Adriana Santos Soprano, Priscila Oliveira de Giuseppe, Hugo Massayoshi Shimo, Tatiani Brenelli Lima, Fernanda Aparecida Heleno Batista, Germanna Lima Righetto, Jose Geraldo de Carvalho Pereira, Daniela Campos Granato, Andrey Fabricio Ziem Nascimento, Fabio Cesar Gozzo, Paulo Sergio Lopes de...
Adriana Santos Soprano, Priscila Oliveira de Giuseppe, Hugo Massayoshi Shimo, Tatiani Brenelli Lima, Fernanda Aparecida Heleno Batista, Germanna Lima Righetto, Jose Geraldo de Carvalho Pereira, Daniela Campos Granato, Andrey Fabricio Ziem Nascimento, Fabio Cesar Gozzo, Paulo Sergio Lopes de Oliveira, Ana Carolina Migliorini Figueira, Juliana Helena Costa Smetana, Adriana Franco Paes Leme, Mario Tyago Murakami, Celso Eduardo Benedetti
Crystal structure and regulation of the citrus Pol III repressor MAF1 by auxin and phosphorylation
Adriana Santos Soprano, Priscila Oliveira de Giuseppe, Hugo Massayoshi Shimo, Tatiani Brenelli Lima, Fernanda Aparecida Heleno Batista, Germanna Lima Righetto, Jose Geraldo de Carvalho Pereira, Daniela Campos Granato, Andrey Fabricio Ziem Nascimento, Fabio Cesar Gozzo, Paulo Sergio Lopes de...
Adriana Santos Soprano, Priscila Oliveira de Giuseppe, Hugo Massayoshi Shimo, Tatiani Brenelli Lima, Fernanda Aparecida Heleno Batista, Germanna Lima Righetto, Jose Geraldo de Carvalho Pereira, Daniela Campos Granato, Andrey Fabricio Ziem Nascimento, Fabio Cesar Gozzo, Paulo Sergio Lopes de Oliveira, Ana Carolina Migliorini Figueira, Juliana Helena Costa Smetana, Adriana Franco Paes Leme, Mario Tyago Murakami, Celso Eduardo Benedetti
Fontes
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Structure Vol. 25, no. 9 (Sept., 2017), p. 1360-1370 |