Effective killing of bacteria under blue-light irradiation promoted by green synthesized silver nanoparticles loaded on reduced graphene oxide sheets
Cynthia S. A. Caires, Luiz A. S. Farias, Luiz E. Gomes, Bruno P. Pinto, Daniel A. Gonçalves, Luiz F. Zagonel, Valter A. Nascimento, Diego C. B. Alves, Ian Colbeck, Corinne Whitby, Anderson R. L. Caires, Heberton Wender
ARTIGO
Inglês
Agradecimentos: This study was supported by Universidade Federal de Mato Grosso do Sul – UFMS/MEC – Brasil and also financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. The authors acknowledge the financial support provided by the...
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Agradecimentos: This study was supported by Universidade Federal de Mato Grosso do Sul – UFMS/MEC – Brasil and also financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. The authors acknowledge the financial support provided by the CAPES-PrInt funding program (grant numbers: 88887.353061/2019-00, 88887.363131/2019-00, 88881.311921/2018-01 and 88887.311920/2018-00) and the National Institute of Science and Technology of Basic Optics and Optics Applied to Life Science (grant number: 465360/2014-9). The authors are grateful to CNPq, CAPES and FUNDECT, Brazilian funding agencies, for their financial support. The authors also would like to acknowledge C.M. Silva, A.R. Lima, L.O. Araujo, L.M. Alves, and F. Benyahia for their technical support, and Professor Renato Gonçalves (IFSC-USP) for providing access to the XPS and micro-Raman equipment
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Abstract: Graphene oxide (GO) materials loaded with silver nanoparticles (AgNPs) have drawn considerable attention due to their capacity to efficiently inactivate bacteria though a multifaceted mechanism of action, as well as for presenting a synergetic effect against bacteria when compared to the...
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Abstract: Graphene oxide (GO) materials loaded with silver nanoparticles (AgNPs) have drawn considerable attention due to their capacity to efficiently inactivate bacteria though a multifaceted mechanism of action, as well as for presenting a synergetic effect against bacteria when compared to the activity of AgNPs and GO alone. In this investigation, we present an inexpensive and environmentally-friendly method for synthesizing reduced GO sheets coated with silver nanoparticles (AgNPs/r-GO) using a coffee extract solution as a green reducing agent. The physical and chemical properties of the produced materials were extensively characterized by scanning electron microscopy (SEM), field-emission gun transmission electron microscopy (FEG-TEM), ultraviolet and visible absorption (UV-Vis), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), inductively coupled plasma-optical emission spectroscopy (ICP-OES) and ion release determination. The results demonstrated that AgNPs/r-GO composites were successfully produced, revealing the formation of micrometer-sized r-GO sheets decorated by AgNPs of approximately 70 nm diameter. Finally, bactericidal and photobactericidal effects of the AgNPs/r-GO composites were tested against Staphylococcus aureus, in which the results showed that the composites presented antimicrobial and photoantimicrobial activities. Moreover, our results demonstrated for the first time, to our knowledge, that an efficient process of bacterial inactivation can be achieved by using AgNPs/r-GO composites under blue light irradiation as a result of three different bacterial killing processes: (i) chemical effect promoted by Ag+ ion release from AgNPs; (ii) photocatalytic activity induced by AgNPs/r-GO composites, enhancing the bacterial photoinactivation due to the excited-Plasmons of the AgNPs when anchored on r-GO; and (iii) photodynamic effect produced by bacterial endogenous photosensitizers under blue-light irradiation. In summary, the present findings demonstrated that AgNPs/r-GO can be obtained by a non-toxic procedure with great potential for biomedical-related applications
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COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
001; 88887.353061/2019-00; 88887.363131/2019-00; 88881.311921/2018-01; 88887.311920/2018-00
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
65360/2014-9
Fechado
Effective killing of bacteria under blue-light irradiation promoted by green synthesized silver nanoparticles loaded on reduced graphene oxide sheets
Cynthia S. A. Caires, Luiz A. S. Farias, Luiz E. Gomes, Bruno P. Pinto, Daniel A. Gonçalves, Luiz F. Zagonel, Valter A. Nascimento, Diego C. B. Alves, Ian Colbeck, Corinne Whitby, Anderson R. L. Caires, Heberton Wender
Effective killing of bacteria under blue-light irradiation promoted by green synthesized silver nanoparticles loaded on reduced graphene oxide sheets
Cynthia S. A. Caires, Luiz A. S. Farias, Luiz E. Gomes, Bruno P. Pinto, Daniel A. Gonçalves, Luiz F. Zagonel, Valter A. Nascimento, Diego C. B. Alves, Ian Colbeck, Corinne Whitby, Anderson R. L. Caires, Heberton Wender
Fontes
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Materials science and engineering. C, Materials for biological applications Vol. 113 (Aug., 2020), n. art. 110984, p. 1-14 |