A genome-wide association study identified loci for yield component traits in sugarcane (Saccharum spp.)A genome-wide association study identified loci for yield component traits in sugarcane (Saccharum spp.)
Fernanda Zatti Barreto, Joao Ricardo Bachega Feijo Rosa, Thiago Willian Almeida Balsalobre, Maria Marta Pastina, Renato Rodrigues Silva, Hermann Paulo Hoffmann, Anete Pereira de Souza, Antonio Augusto Franco Garcia, Monalisa Sampaio Carneiro
ARTIGO
Inglês
Agradecimentos: We gratefully acknowledge Sandro Augusto Ferrarez for providing support in the field experiment. FINEP (Finaciadora de Estudos e Projetos); FAPESP (Fundacao de Amparo a Pesquisa de Sao Paulo)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [08/52197-4]; INCT-Bioetanol...
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Agradecimentos: We gratefully acknowledge Sandro Augusto Ferrarez for providing support in the field experiment. FINEP (Finaciadora de Estudos e Projetos); FAPESP (Fundacao de Amparo a Pesquisa de Sao Paulo)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [08/52197-4]; INCT-Bioetanol (Instituto Nacional de Ciencia e Tecnologia do Bioetanol) [FAPESP 08/57908-6]; INCT-Bioetanol (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq))National Council for Scientific and Technological Development (CNPq) [574002/2008-1]; CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)CAPES [001]; FAPESPFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [10/50091-4, 10/06702-9]; CNPqNational Council for Scientific and Technological Development (CNPq)
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Abstract: Sugarcane (Saccharum spp.) has a complex genome with variable ploidy and frequent aneuploidy, which hampers the understanding of phenotype and genotype relations. Despite this complexity, genome-wide association studies (GWAS) may be used to identify favorable alleles for target traits in...
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Abstract: Sugarcane (Saccharum spp.) has a complex genome with variable ploidy and frequent aneuploidy, which hampers the understanding of phenotype and genotype relations. Despite this complexity, genome-wide association studies (GWAS) may be used to identify favorable alleles for target traits in core collections and then assist breeders in better managing crosses and selecting superior genotypes in breeding populations. Therefore, in the present study, we used a diversity panel of sugarcane, called the Brazilian Panel of Sugarcane Genotypes (BPSG), with the following objectives: (i) estimate, through a mixed model, the adjusted means and genetic parameters of the five yield traits evaluated over two harvest years; (ii) detect population structure, linkage disequilibrium (LD) and genetic diversity using simple sequence repeat (SSR) markers; (iii) perform GWAS analysis to identify marker-trait associations (MTAs); and iv) annotate the sequences giving rise to SSR markers that had fragments associated with target traits to search for putative candidate genes. The phenotypic data analysis showed that the broad-sense heritability values were above 0.48 and 0.49 for the first and second harvests, respectively. The set of 100 SSR markers produced 1,483 fragments, of which 99.5% were polymorphic. These SSR fragments were useful to estimate the most likely number of subpopulations, found to be four, and the LD in BPSG, which was stronger in the first 15 cM and present to a large extension (65 cM). Genetic diversity analysis showed that, in general, the clustering of accessions within the subpopulations was in accordance with the pedigree information. GWAS performed through a multilocus mixed model revealed 23 MTAs, six, three, seven, four and three for soluble solid content, stalk height, stalk number, stalk weight and cane yield traits, respectively. These MTAs may be validated in other populations to support sugarcane breeding programs with introgression of favorable alleles and marker-assisted selection
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FINANCIADORA DE ESTUDOS E PROJETOS - FINEP
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
001
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
08/57908-6; 08/52197-4; 10/50091-4; 10/06702-9
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
574002/2008-1
Aberto
A genome-wide association study identified loci for yield component traits in sugarcane (Saccharum spp.)A genome-wide association study identified loci for yield component traits in sugarcane (Saccharum spp.)
Fernanda Zatti Barreto, Joao Ricardo Bachega Feijo Rosa, Thiago Willian Almeida Balsalobre, Maria Marta Pastina, Renato Rodrigues Silva, Hermann Paulo Hoffmann, Anete Pereira de Souza, Antonio Augusto Franco Garcia, Monalisa Sampaio Carneiro
A genome-wide association study identified loci for yield component traits in sugarcane (Saccharum spp.)A genome-wide association study identified loci for yield component traits in sugarcane (Saccharum spp.)
Fernanda Zatti Barreto, Joao Ricardo Bachega Feijo Rosa, Thiago Willian Almeida Balsalobre, Maria Marta Pastina, Renato Rodrigues Silva, Hermann Paulo Hoffmann, Anete Pereira de Souza, Antonio Augusto Franco Garcia, Monalisa Sampaio Carneiro
Fontes
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PLoS one Vol. 14, no. 7 (July, 2019), n. art. e0219843 |