Comparative genome analysis of phyllosticta citricarpa and phyllosticta capitalensis, two fungi species that share the same host
Carolina Munari Rodrigues, Marco Aurelio Takita, Nicholas Vinicius Silva, Marcelo Ribeiro-Alves, Marcos Antonio Machado
ARTIGO
Inglês
Agradecimentos: The authors thank Inaiara de Souza Pacheco for performing the DNA extraction of the fungi P. citricarpa and P. capitalensis for the execution of this work. We also thank Dr. Chirlei Glienke for ceding the isolates of these fungi to this work
Abstract: Citrus are among the most important crops in the world. However, there are many diseases that affect Citrus caused by different pathogens. Citrus also hosts many symbiotic microorganisms in a relationship that may be advantageous for both organisms. The fungi Phyllosticta citricarpa,...
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Abstract: Citrus are among the most important crops in the world. However, there are many diseases that affect Citrus caused by different pathogens. Citrus also hosts many symbiotic microorganisms in a relationship that may be advantageous for both organisms. The fungi Phyllosticta citricarpa, responsible for citrus black spot, and Phyllosticta capitalensis, an endophytic species, are examples of closely related species with different behavior in citrus. Both species are always biologically associated and are morphologically very similar, and comparing their genomes could help understanding the different lifestyles. In this study, a comparison was carried to identify genetic differences that could help us to understand the biology of P. citricarpa and P. capitalensis.ResultsDrafts genomes were assembled with sizes close to 33Mb for both fungi, carrying 15,206 and 14,797 coding sequences for P. citricarpa and P. capitalensis, respectively. Even though the functional categories of these coding sequences is similar, enrichment analysis showed that the pathogenic species presents growth and development genes that may be necessary for the pathogenicity of P. citricarpa. On the other hand, family expansion analyses showed the plasticity of the genome of these species. Particular families are expanded in the genome of an ancestor of P. capitalensis and a recent expansion can also be detected among this species. Additionally, evolution could be driven by environmental cues in P. citricarpa.ConclusionsThis work demonstrated genomic differences between P. citricarpa and P. capitalensis. Although the idea that these differences could explain the different lifestyles of these fungi, we were not able to confirm this hypothesis. Genome evolution seems to be of real importance among the Phyllosticta isolates and it is leading to different biological characteristics of these species
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CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
560443/2010–2; 163021/2011–2; 573848/2008–4; 465440/2014–2
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
08/57909–2; 14/50880–0
Aberto
Comparative genome analysis of phyllosticta citricarpa and phyllosticta capitalensis, two fungi species that share the same host
Carolina Munari Rodrigues, Marco Aurelio Takita, Nicholas Vinicius Silva, Marcelo Ribeiro-Alves, Marcos Antonio Machado
Comparative genome analysis of phyllosticta citricarpa and phyllosticta capitalensis, two fungi species that share the same host
Carolina Munari Rodrigues, Marco Aurelio Takita, Nicholas Vinicius Silva, Marcelo Ribeiro-Alves, Marcos Antonio Machado
Fontes
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BMC genomics Vol. 20 (July, 2019), n. art. 554 |