Amino acid restriction increases ß-cell death under challenging conditions
Luciana Mateus Goncalves, Jean Franciesco Vettorazzi, Emerielle Cristine Vanzela, Mariana Sarto Figueiredo, Thiago Martins Batista, Claudio Cesar Zoppi, Antonio Carlos Boschero, Everardo Magalhaes Carneiro
ARTIGO
Inglês
[Amino acid restriction increases beta-cell death under challenging conditions]
Agradecimentos: The authors thank M. Carnelossi for her assistance. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil (CAPES - Finance Code 001) and by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2014/01717-9)
Abstract: Malnutrition programs metabolism, favor dysfunction of beta cells. We aimed to establish an in vitro protocol of malnutrition, assessing the effect of amino acid restriction upon the beta cells. Insulin-producing cells INS-1E and pancreatic islets were maintained in RPMI 1640 medium...
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Abstract: Malnutrition programs metabolism, favor dysfunction of beta cells. We aimed to establish an in vitro protocol of malnutrition, assessing the effect of amino acid restriction upon the beta cells. Insulin-producing cells INS-1E and pancreatic islets were maintained in RPMI 1640 medium containing 1x (Ctl) or 0.25x (AaR) of amino acids. We evaluated several markers of beta-cell function and viability. AaR Insulin secretion was reduced, whereas cell viability was unaltered. Calcium oscillations in response to glucose increased in AaR. AaR showed lower Ins1 RNAm, snap 25, and PKC (protein kinase C) protein content, whereas phospho-eIF2 alpha was increased. AaR cells exposed to nutrient or chemical challenges displayed higher apoptosis rates. We showed that amino acid restriction programmed beta cell and induced functional changes. This model might be useful for the study of molecular mechanisms involved with beta-cell programming helping to establish novel therapeutic targets to prevent harmful outcomes of malnutrition
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COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
001
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2014/01717-9
Fechado
Amino acid restriction increases ß-cell death under challenging conditions
Luciana Mateus Goncalves, Jean Franciesco Vettorazzi, Emerielle Cristine Vanzela, Mariana Sarto Figueiredo, Thiago Martins Batista, Claudio Cesar Zoppi, Antonio Carlos Boschero, Everardo Magalhaes Carneiro
Amino acid restriction increases ß-cell death under challenging conditions
Luciana Mateus Goncalves, Jean Franciesco Vettorazzi, Emerielle Cristine Vanzela, Mariana Sarto Figueiredo, Thiago Martins Batista, Claudio Cesar Zoppi, Antonio Carlos Boschero, Everardo Magalhaes Carneiro
Fontes
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Journal of cellular physiology v. 234, n. 10, p. 16679-16684, Oct. 2019 |