Feulgen-DNA absorption curves of polytene chromosome regions of Rhynchosciara americana
Maria Luiza S. Mello
ARTIGO
Inglês
Feulgen absorption spectra of polytene chromosome regions, differing in types of DNA composition and of chromatin compactness, were determined microspectrophotometrically in squashed salivary glands ofhynchosciara americana. The absorption curves exhibited a secondary maximum at their ascending...
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Feulgen absorption spectra of polytene chromosome regions, differing in types of DNA composition and of chromatin compactness, were determined microspectrophotometrically in squashed salivary glands ofhynchosciara americana. The absorption curves exhibited a secondary maximum at their ascending branch after the chromatins were maximally depurinated. The degree of shoulder prominence could not be specifically correlated with the packing state or even with richness in satellite repetitive DNA of the chromatins. It is assumed that other factors may exist which play a part in the phenomenon that gives rise to the Feulgen absorption shoulder characteristics. Differences in the extraction rate of the apurinic acid fragments due to their binding to specific non-histone proteins are suggested as contributing to the above-mentioned absorption patterns when comparing the various chromatin types
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Feulgen absorption spectra of polytene chromosome regions, differing in types of DNA composition and of chromatin compactness, were determined microspectrophotometrically in squashed salivary glands of hynchosciara americana. The absorption curves exhibited a secondary maximum at their ascending...
Ver mais
Feulgen absorption spectra of polytene chromosome regions, differing in types of DNA composition and of chromatin compactness, were determined microspectrophotometrically in squashed salivary glands of hynchosciara americana. The absorption curves exhibited a secondary maximum at their ascending branch after the chromatins were maximally depurinated. The degree of shoulder prominence could not be specifically correlated with the packing state or even with richness in satellite repetitive DNA of the chromatins. It is assumed that other factors may exist which play a part in the phenomenon that gives rise to the Feulgen absorption shoulder characteristics. Differences in the extraction rate of the apurinic acid fragments due to their binding to specific non-histone proteins are suggested as contributing to the above-mentioned absorption patterns when comparing the various chromatin types
Ver menos
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
1975/827; 1976/735
Fechado
DOI: https://doi.org/10.1007/BF01011924
Texto completo: https://link.springer.com/article/10.1007/BF01011924
Feulgen-DNA absorption curves of polytene chromosome regions of Rhynchosciara americana
Maria Luiza S. Mello
Feulgen-DNA absorption curves of polytene chromosome regions of Rhynchosciara americana
Maria Luiza S. Mello
Fontes
Histochemical journal (Fonte avulsa) |