In vivo genotoxicity of a commercial C.I. disperse red 1 dye
Fábio Henrique Fernandes, Mário Otávio Botasso-Nasciutti, André Luiz Ventura Sávio, Leonardo da Cunha Menezes Souza, Jhennifer Rebecca Fernandes-Cal, Fábio Florença Cardoso, Marcos Roberto de Mattos Fontes, Anjaína Fernandes Albuquerque, Carla C. Munari, Fábio Kummrow, Gisela de Aragão Umbuzeiro,...
Fábio Henrique Fernandes, Mário Otávio Botasso-Nasciutti, André Luiz Ventura Sávio, Leonardo da Cunha Menezes Souza, Jhennifer Rebecca Fernandes-Cal, Fábio Florença Cardoso, Marcos Roberto de Mattos Fontes, Anjaína Fernandes Albuquerque, Carla C. Munari, Fábio Kummrow, Gisela de Aragão Umbuzeiro, Daisy Maria Fávero Salvadori
ARTIGO
Inglês
Agradecimentos: The authors are grateful to Paulo Cesar Georgete for technical assistance. Sponsors: Coordination for the Improvement of Higher Education Personnel - CAPES. Brazilian National Council for Scientific and Technological Development - CNPq; grant number: 465571/2014-0. São Paulo Research...
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Agradecimentos: The authors are grateful to Paulo Cesar Georgete for technical assistance. Sponsors: Coordination for the Improvement of Higher Education Personnel - CAPES. Brazilian National Council for Scientific and Technological Development - CNPq; grant number: 465571/2014-0. São Paulo Research Foundation - FAPESP; grant numbers: 2014/50945-4; 2008/10449-7
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Abstract: Color Index (C.I.) Disperse Red 1 (DR1) is a widely used textile azo dye found in rivers. As it may not be completely removed by conventional treatments, humans can be exposed through drinking water. Studies have supported in vitro toxicity and mutagenicity of commercial DR1. This study...
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Abstract: Color Index (C.I.) Disperse Red 1 (DR1) is a widely used textile azo dye found in rivers. As it may not be completely removed by conventional treatments, humans can be exposed through drinking water. Studies have supported in vitro toxicity and mutagenicity of commercial DR1. This study aimed to investigate the mutagenic and toxicogenomic effects of commercial DR1 in multiple tissues/organs of Swiss male mice. For that, animals were orally exposed to the dye (by gavage), at single doses of 0.0005, 0.005, 0.5, 50, or 500 mg/kg bw. The two lowest doses were equivalent to the ones found in two Brazilian rivers receiving influx of textile discharges. Cytotoxicity, micronucleated cell frequencies (for all doses tested), primary DNA damage (comet assay), and gene expression profiling of (0.0005 and 0.005 mg/kg of bw) were investigated 24 hr after animal exposure to commercial DR1. Data showed increased frequencies of micronucleated polychromatic erythrocytes in bone marrow cells after treatment with 0.5 and 50 mg/kg bw. At 0.005 mg/kg bw, commercial DR1 induced an increase of primary DNA damage in liver, but not in kidney cells. Additionally, upregulation of genes involved in the inflammatory process (IL1B) (0.0005 and 0.005 mg/kg bw) and cell-cycle control (CDKN1A) in liver cells, and apoptosis (BCL2 and BAX) in leukocytes (0.005 mg/kg bw) were also detected. In conclusion, the commercial DR1 was genotoxic (chromosome aberrations and primary DNA damage) and modulated gene expression in mice, and such effects were dependent on the doses and tissues analyzed
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CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
465571/2014-0
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2008/10449-7; 2014/50945-4
Fechado
DOI: https://doi.org/10.1002/em.22226
Texto completo: https://onlinelibrary.wiley.com/doi/10.1002/em.22226
In vivo genotoxicity of a commercial C.I. disperse red 1 dye
Fábio Henrique Fernandes, Mário Otávio Botasso-Nasciutti, André Luiz Ventura Sávio, Leonardo da Cunha Menezes Souza, Jhennifer Rebecca Fernandes-Cal, Fábio Florença Cardoso, Marcos Roberto de Mattos Fontes, Anjaína Fernandes Albuquerque, Carla C. Munari, Fábio Kummrow, Gisela de Aragão Umbuzeiro,...
Fábio Henrique Fernandes, Mário Otávio Botasso-Nasciutti, André Luiz Ventura Sávio, Leonardo da Cunha Menezes Souza, Jhennifer Rebecca Fernandes-Cal, Fábio Florença Cardoso, Marcos Roberto de Mattos Fontes, Anjaína Fernandes Albuquerque, Carla C. Munari, Fábio Kummrow, Gisela de Aragão Umbuzeiro, Daisy Maria Fávero Salvadori
In vivo genotoxicity of a commercial C.I. disperse red 1 dye
Fábio Henrique Fernandes, Mário Otávio Botasso-Nasciutti, André Luiz Ventura Sávio, Leonardo da Cunha Menezes Souza, Jhennifer Rebecca Fernandes-Cal, Fábio Florença Cardoso, Marcos Roberto de Mattos Fontes, Anjaína Fernandes Albuquerque, Carla C. Munari, Fábio Kummrow, Gisela de Aragão Umbuzeiro,...
Fábio Henrique Fernandes, Mário Otávio Botasso-Nasciutti, André Luiz Ventura Sávio, Leonardo da Cunha Menezes Souza, Jhennifer Rebecca Fernandes-Cal, Fábio Florença Cardoso, Marcos Roberto de Mattos Fontes, Anjaína Fernandes Albuquerque, Carla C. Munari, Fábio Kummrow, Gisela de Aragão Umbuzeiro, Daisy Maria Fávero Salvadori
Fontes
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Environmental and molecular mutagenesis v. 59, n. 9, p. 822-828, Dec. 2018 |