Nanofilms of hyaluronan/chitosan assembled layer-by-layer : An antibacterial surface for Xylella fastidiosa
Jacobo Hernández-Montelongo, Vicente F. Nascimento, Duber Murillo, Thiago B. Taketa, Prasana Sahoo, Alessandra A. de Souza, Marisa M. Beppu and Monica A. Cotta
ARTIGO
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Agradecimentos: The authors acknowledge the support from Analytical Resources and Calibration Laboratory (LRAC) from Chemical Engineering Department and the Multi-User Lab (LAMULT) from Institute of Physics "Gleb Wataghin", both at UNICAMP, which provided invaluable access to their equipments. We...
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Agradecimentos: The authors acknowledge the support from Analytical Resources and Calibration Laboratory (LRAC) from Chemical Engineering Department and the Multi-User Lab (LAMULT) from Institute of Physics "Gleb Wataghin", both at UNICAMP, which provided invaluable access to their equipments. We would also like to thank the National Nanotechnology Laboratory (LNNano) for granting access to the electron microscopy facilities. This work was financially supported by FAPESP (grant numbers 2010/51748-7 and 2013/02300-1), CNPq and CAPES. J. Hernandez-Montelongo and P. Sahoo acknowledge FAPESP scholarships; FAPEAM and CAPES provide the scholarships to V. Nascimento and D. Murillo, respectively
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Abstract: In this work, nanofilms of hyaluronan/chitosan (HA/CHI) assembled layer by layer were synthesized; their application as a potential antimicrobial material was demonstrated for the phytopathogen Xylella fastidiosa, a gram-negative bacterium, here used as a model. For the synthesis, the...
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Abstract: In this work, nanofilms of hyaluronan/chitosan (HA/CHI) assembled layer by layer were synthesized; their application as a potential antimicrobial material was demonstrated for the phytopathogen Xylella fastidiosa, a gram-negative bacterium, here used as a model. For the synthesis, the influence of pH and ionic strength of these natural polymer stem-solutions on final characteristics of the HA/CHI nanofilms was studied in detail. The antibacterial effect was evaluated using widefield fluorescence microscopy. These results were correlated with the chemical properties of the nanofilms, studied by FTIR and Raman spectroscopy, as well as with their morphology and surface properties characterized using SEM and AFM. The present findings can be extended to design and optimize HA/CHI nanofilms with enhanced antimicrobial behavior for other type of phytopathogenic gram-negative bacteria species, such as Xanthomonas citri, Xanthomas campestri and Ralstonia solanacearum
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2010/51748-7; 2013/02300-1
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DO AMAZONAS - FAPEAM
Fechado
Nanofilms of hyaluronan/chitosan assembled layer-by-layer : An antibacterial surface for Xylella fastidiosa
Jacobo Hernández-Montelongo, Vicente F. Nascimento, Duber Murillo, Thiago B. Taketa, Prasana Sahoo, Alessandra A. de Souza, Marisa M. Beppu and Monica A. Cotta
Nanofilms of hyaluronan/chitosan assembled layer-by-layer : An antibacterial surface for Xylella fastidiosa
Jacobo Hernández-Montelongo, Vicente F. Nascimento, Duber Murillo, Thiago B. Taketa, Prasana Sahoo, Alessandra A. de Souza, Marisa M. Beppu and Monica A. Cotta
Fontes
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Carbohydrate polymers: a journal devoted to scientific and technological aspects of industrially relevant polysaccharides Vol. 136 (2016), p. 1-11 |