Perfusion microfermentor integrated into a fiber optic quasi-elastic light scattering sensor for fast screening of microbial growth parameters
Marco César Prado Soares, Franciele Flores, Carlos Kenichi Suzuki, Lucimara Gaziola de la Torre, Eric Fujiwara
ARTIGO
Inglês
Agradecimentos: This work was supported in part by the FAPESP under grant 2017/20445-8, and CAPES—Finance code 001;We thank José Alberto Fracassi da Silva (Institute of Chemistry—IQ/Unicamp) for the laser ablation machine, and the Microfabrication Laboratory of the Brazilian Nanotechnology National...
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Agradecimentos: This work was supported in part by the FAPESP under grant 2017/20445-8, and CAPES—Finance code 001;We thank José Alberto Fracassi da Silva (Institute of Chemistry—IQ/Unicamp) for the laser ablation machine, and the Microfabrication Laboratory of the Brazilian Nanotechnology National Laboratory (LNNano), CNPEM/MCTI, for the fabrication of the bioreactor’s glasses
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This research presents a microfermentor integrated into an optical fiber sensor based on quasi-elastic light scattering (QELS) to monitor and swiftly identify cellular growth kinetic parameters. The system uses a 1310 nm laser light that is guided through single-mode silica optical fibers to the...
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This research presents a microfermentor integrated into an optical fiber sensor based on quasi-elastic light scattering (QELS) to monitor and swiftly identify cellular growth kinetic parameters. The system uses a 1310 nm laser light that is guided through single-mode silica optical fibers to the interior of perfusion chambers, which are separated by polycarbonate membranes (470 nm pores) from microchannels, where a culture medium flows in a constant concentration. The system contains four layers, a superior and an inferior layer made of glass, and two intermediate poly(dimethylsiloxane) layers that contain the microchannels and the perfusion chambers, forming a reversible microfluidic device that requires only the sealing of the fibers to the inferior glass cover. The QELS autocorrelation decay rates of the optical signals were correlated to the cells counting in a microscope, and the application of this microsystem to the monitoring of alcoholic fermentation of Saccharomyces cerevisiae resulted in the kinetic parameters of KM = 4.1 g/L and μm = 0.49 h−1. These results agree with both the data reported in the literature and with the control batch test, showing that it is a reliable and efficient biological monitoring system.
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COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2017/20445-8
Aberto
DOI: https://doi.org/10.3390/s19112493
Texto completo: https://www.mdpi.com/1424-8220/19/11/2493/htm
Perfusion microfermentor integrated into a fiber optic quasi-elastic light scattering sensor for fast screening of microbial growth parameters
Marco César Prado Soares, Franciele Flores, Carlos Kenichi Suzuki, Lucimara Gaziola de la Torre, Eric Fujiwara
Perfusion microfermentor integrated into a fiber optic quasi-elastic light scattering sensor for fast screening of microbial growth parameters
Marco César Prado Soares, Franciele Flores, Carlos Kenichi Suzuki, Lucimara Gaziola de la Torre, Eric Fujiwara
Fontes
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Sensors Vol. 19, no. 11 (May, 2019), n. art. 2493 |