Inflammatory responses induced in mice by lectin from Talisia esculenta seeds
M.L.R. Macedo, M.G.M. Freire, I.A. De Souza, A.C.M. Silva, M.S. Lima, S. Marangoni, E. Antunes, W.M.S.C. Tamashiro
ARTIGO
Inglês
Agradecimentos: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and CNPq
A novel lectin from Talisia esculenta seeds (TEL) has recently been purified and characterized. In this study we investigated the proinflammatory activity of TEL in mice using both the air-pouch and peritoneal cavity as well as paw oedema models. TEL (10-40 mug) induced significant neutrophil and...
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A novel lectin from Talisia esculenta seeds (TEL) has recently been purified and characterized. In this study we investigated the proinflammatory activity of TEL in mice using both the air-pouch and peritoneal cavity as well as paw oedema models. TEL (10-40 mug) induced significant neutrophil and mononuclear cell recruitment when injected into either mouse air-pouch or peritoneal cavity. The neutrophil accumulation into the air-pouch was dose- and time-dependent with a maximal response at 16 h. returning to control levels at 72 h whereas maximal mononuclear cell accumulation was observed at 24 h after TEL injection. The same profile of neutrophil accumulation was observed when this lectin was injected into mouse peritonea] cavity, although the maximal mononuclear cell recruitment was observed 48 h after TEL injection. Additionally, TEL (12.5-200 mug/paw) caused a dose-dependent mice paw, as evaluated at 4 h after the lectin injection. D-mannose, better than D-glucose, significantly inhibited TEL-induced neutrophil migration into the peritoneal cavity or air-pouch. D-galactose had no effect on TEL-induced neutrophil migration in either cavity studied. On the other hand, D-mannose slightly inhibited the TEL-induced paw oedema. whereas neither D-glucose nor D-galactose affected this phenomenon. In conclusion, our data show that TEL induces neutrophil and mononuclear cell accumulation by a mechanism related to their specific sugar-binding properties
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
Fechado
Inflammatory responses induced in mice by lectin from Talisia esculenta seeds
M.L.R. Macedo, M.G.M. Freire, I.A. De Souza, A.C.M. Silva, M.S. Lima, S. Marangoni, E. Antunes, W.M.S.C. Tamashiro
Inflammatory responses induced in mice by lectin from Talisia esculenta seeds
M.L.R. Macedo, M.G.M. Freire, I.A. De Souza, A.C.M. Silva, M.S. Lima, S. Marangoni, E. Antunes, W.M.S.C. Tamashiro
Fontes
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Toxicon: an interdisciplinary journal on the toxins derived from animals, plants and microorganisms Vol. 42, no. 3 (Sept., 2003), p. 275-280 |