Pharmacological characterization of the rat paw edema induced by Bothrops lanceolatus (Fer de lance) venom
C. Bon, A.L. de Araujo, E. Antunes, L. de Faria
ARTIGO
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The inflammatory response induced by Bothrops lanceolatus venom (BLV) in the rat hind-paw was studied measuring paw edema. Non-heated BLV (75 mug/paw) caused a marked paw edema accompanied by intense haemorrhage whereas heated venom (97 degreesC, 30 s; 12.5-100 mug/paw) produced a dose- and...
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The inflammatory response induced by Bothrops lanceolatus venom (BLV) in the rat hind-paw was studied measuring paw edema. Non-heated BLV (75 mug/paw) caused a marked paw edema accompanied by intense haemorrhage whereas heated venom (97 degreesC, 30 s; 12.5-100 mug/paw) produced a dose- and time-dependent non-haemorrhage edema. The response with heated BLV was maximal within 15 min disappearing over 24 h. Heated venom was then routinely used at the dose of 75 mug/paw. The prostacyclin analogue iloprost (0.1 mug/paw) potentiated by 125% the venom-induced edema. The histamine H-1 receptor antagonist mepyramine (6 mg/kg) or the serotonin/histamine receptor antagonist cyproheptadine (6 mg/kg) partially inhibited BLV-induced edema whereas the combination of both compounds virtually abolished the edema. The lipoxygenase inhibitor BWA4C (10 mg/kg), but not the cyclooxygenase inhibitor indomethacin (10 mg/kg), significantly inhibited the edema (35% reduction; P < 0.05). Dexamethasone (1 mg/kg) also markedly (P < 0.001) reduced venom-induced edema. The brady-kinin B-2 receptor antagonist Hoe 140 (0.6 mg/kg) reduced by 30% (P < 0.05) the venom induced edema, whereas the angiotensin-converting enzyme inhibitor captopril (300 <mu>g/paw) potentiated by 42% (P < 0.05) the edema. Bothrops lanceolatus antivenon (anti-BLV) reduced by 28% (P < 0.05) the venom-induced edema while intravenous administration of antivenom failed to affect the edema. In conclusion, BLV-induced rat paw edema involves mast cell degranulation causing local release of histamine and serotonin, a phenomenon mediated mainly by kinins and lipoxygenase metabolites. Additionally, the use of a specific Bothrops lanceolatus antivenom, given subplantarily or intravenously, revealed to be little effective to prevent BLV-induced edema
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
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Pharmacological characterization of the rat paw edema induced by Bothrops lanceolatus (Fer de lance) venom
C. Bon, A.L. de Araujo, E. Antunes, L. de Faria
Pharmacological characterization of the rat paw edema induced by Bothrops lanceolatus (Fer de lance) venom
C. Bon, A.L. de Araujo, E. Antunes, L. de Faria
Fontes
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Toxicon: an interdisciplinary journal on the toxins derived from animals, plants and microorganisms Vol. 39, no. 6 (June, 2001), p. 825-830 |