MicroRNA profile of pediatric pilocytic astrocytomas identifies two tumor-specific signatures when compared to non-neoplastic white matter
Luiz Guilherme Darrigo Junior, Regia Caroline Peixoto Lira, Paola Fernanda Fedatto, David Santos Marco Antonio, Elvis Terci Valera, Simone Aguiar, Jose Andres Yunes, Silvia Regina Brandalise, Luciano Neder, Fabiano Pinto Saggioro, Aline Paixao Becker, Ricardo Santos de Oliveira, Helio Rubens...
Luiz Guilherme Darrigo Junior, Regia Caroline Peixoto Lira, Paola Fernanda Fedatto, David Santos Marco Antonio, Elvis Terci Valera, Simone Aguiar, Jose Andres Yunes, Silvia Regina Brandalise, Luciano Neder, Fabiano Pinto Saggioro, Aline Paixao Becker, Ricardo Santos de Oliveira, Helio Rubens Machado, Rodrigo Alexandre Panepucci, Luiz Gonzaga Tone, Carlos Alberto Scrideli
ARTIGO
Inglês
PurposesPilocytic astrocytoma (PA) is a low-grade neoplasm frequently found in childhood. PA is characterized by slow growth and a relatively good prognosis. Genetic mechanisms such as activation of MAPK, BRAF gene deregulation and neurofibromatosis type 1 (NF1) syndrome have been associated with PA...
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PurposesPilocytic astrocytoma (PA) is a low-grade neoplasm frequently found in childhood. PA is characterized by slow growth and a relatively good prognosis. Genetic mechanisms such as activation of MAPK, BRAF gene deregulation and neurofibromatosis type 1 (NF1) syndrome have been associated with PA development. Epigenetic signature and miRNA expression profile are providing new insights about different types of tumor, including PAs.MethodsIn the present study we evaluated global miRNA expression in 16 microdissected pediatric PA specimens, three NF1-associated PAs and 11 cerebral white matter (WM) samples by the microarray method. An additional cohort of 20 PAs was used to validate by qRT-PCR the expression of six miRNAs differentially expressed in the microarray data.ResultsUnsupervised hierarchical clustering analysis distinguished one cluster with nine PAs, including all NF1 cases and a second group consisting of the WM samples and seven PAs. Among 88 differentially expressed miRNAs between PAs and WM samples, the most underexpressed ones regulate classical pathways of tumorigenesis, while the most overexpressed miRNAs are related to pathways such as focal adhesion, P53 signaling pathway and gliomagenesis. The PAs/NF1 presented a subset of underexpressed miRNAs, which was also associated with known deregulated pathways in cancer such as cell cycle and hippo pathway.ConclusionsIn summary, our data demonstrate that PA harbors at least two distinct miRNA signatures, including a subgroup of patients with NF1/PA lesions
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CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
471885/2013-4
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2010/07020-9
Fechado
MicroRNA profile of pediatric pilocytic astrocytomas identifies two tumor-specific signatures when compared to non-neoplastic white matter
Luiz Guilherme Darrigo Junior, Regia Caroline Peixoto Lira, Paola Fernanda Fedatto, David Santos Marco Antonio, Elvis Terci Valera, Simone Aguiar, Jose Andres Yunes, Silvia Regina Brandalise, Luciano Neder, Fabiano Pinto Saggioro, Aline Paixao Becker, Ricardo Santos de Oliveira, Helio Rubens...
Luiz Guilherme Darrigo Junior, Regia Caroline Peixoto Lira, Paola Fernanda Fedatto, David Santos Marco Antonio, Elvis Terci Valera, Simone Aguiar, Jose Andres Yunes, Silvia Regina Brandalise, Luciano Neder, Fabiano Pinto Saggioro, Aline Paixao Becker, Ricardo Santos de Oliveira, Helio Rubens Machado, Rodrigo Alexandre Panepucci, Luiz Gonzaga Tone, Carlos Alberto Scrideli
MicroRNA profile of pediatric pilocytic astrocytomas identifies two tumor-specific signatures when compared to non-neoplastic white matter
Luiz Guilherme Darrigo Junior, Regia Caroline Peixoto Lira, Paola Fernanda Fedatto, David Santos Marco Antonio, Elvis Terci Valera, Simone Aguiar, Jose Andres Yunes, Silvia Regina Brandalise, Luciano Neder, Fabiano Pinto Saggioro, Aline Paixao Becker, Ricardo Santos de Oliveira, Helio Rubens...
Luiz Guilherme Darrigo Junior, Regia Caroline Peixoto Lira, Paola Fernanda Fedatto, David Santos Marco Antonio, Elvis Terci Valera, Simone Aguiar, Jose Andres Yunes, Silvia Regina Brandalise, Luciano Neder, Fabiano Pinto Saggioro, Aline Paixao Becker, Ricardo Santos de Oliveira, Helio Rubens Machado, Rodrigo Alexandre Panepucci, Luiz Gonzaga Tone, Carlos Alberto Scrideli
Fontes
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Journal of Neuro-Oncology Vol. 141, no. 2 (Jan., 2019), p. 373-382 |