Compartmentalization of therapeutic proteins into semi-crystalline PEG-PCL polymersomes
Juliana de Almeida Pachioni-Vasconcelos, Alexsandra Conceição Apolinário, André Moreni Lopes, Adalberto Pessoa, Leandro Ramos Souza Barbosa, Carlota de Oliveira Rangel-Yagui
ARTIGO
Inglês
Polymersomes are self-assembled nanostructures with high loading capacity, possibility to deliver hydrophilic as well as hydrophobic drugs and stealth characteristic resulting in low immunogenicity. These vesicles can be used to deliver enzyme drugs such as L-asparaginase (ASNase), a first line drug...
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Polymersomes are self-assembled nanostructures with high loading capacity, possibility to deliver hydrophilic as well as hydrophobic drugs and stealth characteristic resulting in low immunogenicity. These vesicles can be used to deliver enzyme drugs such as L-asparaginase (ASNase), a first line drug for acute lymphoblastic leukemia treatment. Here, polymersomes based on three semi-crystalline copolymers of poly(ethylene glycol)-b-poly(ε-caprolactone), namely PEG45PCL44, PEG114PCL98, and PEG114PCL114, were investigated for the encapsulation of ASNase, as well as of bovine serum albumin (BSA) as a model protein. Critical aggregation concentration (CAC) of the copolymers was determined by fluorescence spectroscopy and the values varied from 0.6 to 1.26 mg/L. Using film hydration, polymersomes of 200–400 nm and narrow size distribution (polydispersity index values of 0.2–0.3) were obtained when centrifugation was used as a post-film technique. The encapsulation efficiency (EE %) was determined either after centrifugation of the suspension, followed by the proteins measurement in the supernatant or after Size Exclusion Chromatography (SEC) purification, in which the quantification was performed in the eluted fractions corresponding to the free protein. Higher encapsulation efficiency values were obtained after centrifugation (EE% ≈ 20%) in comparison to the measurements after SEC (EE% ≈ 1–5%), indicating that the protein could be partially entrapped in the polymeric network when centrifugation is used as separation method. Nonetheless, this system could provide an initial effect of the free ASNase followed by a long-term effect based on the encapsulated enzyme, leading to decreased dose administration
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FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
2013/08617-7; 2014/10456-4
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
303334/2014-2; 301832/2017-0; 445442/2014-0
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
001
Fechado
Compartmentalization of therapeutic proteins into semi-crystalline PEG-PCL polymersomes
Juliana de Almeida Pachioni-Vasconcelos, Alexsandra Conceição Apolinário, André Moreni Lopes, Adalberto Pessoa, Leandro Ramos Souza Barbosa, Carlota de Oliveira Rangel-Yagui
Compartmentalization of therapeutic proteins into semi-crystalline PEG-PCL polymersomes
Juliana de Almeida Pachioni-Vasconcelos, Alexsandra Conceição Apolinário, André Moreni Lopes, Adalberto Pessoa, Leandro Ramos Souza Barbosa, Carlota de Oliveira Rangel-Yagui
Fontes
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Soft materials (2020) |