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Abstract: We aim is to present a learning approach for students to work on a hands-on project that may be applied to different contexts. This experience relates to an initiative to foster entrepreneurial education embedded in economic sustainability based on the best local practices (highest-ranked municipalities) that we find and analyze to solve complex problems in the business environment. Design/Methodology/Approach: our approach is to collaborate with policymakers at the municipal level via a research-based project approach called Vertically Integrated Project (VIP). In our VIP, students work with instructors in a long-term effort to identify challenges and opportunities by working with the community to analyze problems, develop solutions (with different techniques and approaches), and monitor their implementation. Findings: new learning approaches can engage the students in real-world problem, adding value to their formation and giving back to society the investment they made in the Brazilian public university. Originality: this initiative is in tune with the 4th and the 11th sustainable development goals (SDGs) adopted by the United Nations (UN). Practical implications: this study aims to stimulate entrepreneurial education using multidisciplinary and alternative learning approaches. Social implications: this approach can deliver SDG-related impact to local communities by linking research-based teaching with community outreach

Abstract: CLK2 protein (Cdc2-like kinase) after being phosphorylated by Akt, phosphorylates PGC-1a and consequently reduces the transcription of gluconeogenic genes. Obese and insulin resistant (IR) animals showed lower levels of CLK2 in the liver, which makes this protein an important therapeutic target in the control of glucose homeostasis. On the other hand, the exercise is an effective strategy to reduce IR and control hepatic glucose production. So far, no study has been performed in order to assess the role of exercise in modulating the CLK2 protein in obese animals. Therefore, the aim of this study was to evaluate the role of chronic physical exercise on the levels and phosphorylation of CLK2 in hepatic tissue of obese and IR mice. Twenty four Swiss mice (4 weeks old) were divided into 3 groups (8 animals / group): Sedentary Control (C) sedentary animals fed with control diet, Sedentary Obese (SO) sedentary animals fed with HFD and Trained Obese (TO) animals fed with HFD and submitted to the training protocol. Protocol training was carried out for 1h / day, 5 days / week during 8 weeks and it was performed at the intensity of 60% of maximum power, which was determined at the beginning of the experiment. During the last experimental week the insulin tolerance test (ITT) and glucose tolerance test (GTT) were performed. Twenty four hours after the last exercise session the animals were euthanized and the liver was harvested for subsequent analysis. After the training protocol, TO group improved insulin sensitivity when compared to SO group. This finding was, in part, due to the increased phosphorylation of CLK2 by Akt in the trained animals. Because after occurring the elevation of CLK2 phosphorylation there was increased phosphorylation and nuclear extrusion of FoxO1 and this resulted in a reduced synthesis of gluconeogenic enzymes (G6Pase, PEPCK and PCB) which consequently reduced the hepatic glucose production. Therefore, it can be concluded that physical exercise, through CLK2 protein, reduces hepatic glucose production which collaborate to reduce the development of type 2 diabetes in obese animals