Agradecimentos: We would like to express our gratitude to Mr Roque Zillio at Embrapa for excellent management of the vineyards and assistance in berry harvesting. We also acknowledge the staff at Embrapa for carrying out the juice production. CONFLICT OF INTEREST The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The sponsors had no role in study design; in the collection, analysis and interpretation of data; in the writing of the report; or in the decision to submit the article for publication. The work was supported by a research grant Embrapa/SEG (20.18.01.019.00.00) to PS Ritschel. MOM Marques was the recipient of a productivity grant by the National Council for Scientific and Technological Development, CNPq (309 957/2015-0), HAG Gomez was supported by a Doctoral Students Abroad (PAEDEX/AUIP/PROPG) scholarship from São Paulo State University (UNESP), MJ Carneiro received a scholarship grant Embrapa/CNPq (404 250/2020-3), and GF Niederauer was supported by a PIBITI/CNPq/IAC scholarship (115240/2016-0) Ver menos

Abstract: Yield, disease tolerance, and climate adaptation are important traits in grapevine genetic breeding programs. Selection for these characteristics causes unpredictable changes in primary and specialized metabolism, affecting the physicochemical properties and chemical composition of the berries and their processed products, juice, and wine. In this study, we investigated the influence of the genetic distance between grapevine genotypes on the chemical signatures of the juices, by integrating comprehensive metabolic profiling to genetic analyses. The studied grapevine cultivars exhibited low genetic diversity. Breeding for agronomic traits promoted higher contents of soluble sugars, total phenolics, and anthocyanins in the juices. Untargeted juice metabolomics identified a total of 147 metabolites, consisting of 30 volatiles, 21 phenolics, and 96 ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) features. Juices from grapes of the most recent cultivars exhibited increased levels of trans-resveratrol, catechin, and luteolin. The blend of volatiles from juices of later cultivars was also more complex, consisting of 29 distinct metabolites in 'BRS Magna'. Grapes from 'BRS Carmem', an intermediate cultivar, gave the most divergent UHPLC-MS juice profile. Contents of soluble solids, total phenolics, and anthocyanins in grape juices were increased by controlled crosses and hybrid selection. Integrative analyses demonstrated that the juices' metabolic profiles accurately represent the cultivars' genetic distances. Juices from 'BRS Violeta' and 'BRS Magna' show relevant positive association with health-related phenolics and a distinct set of odor volatiles, although these characteristics were specifically sought by breeding. © 2023 Society of Chemical Industry Ver menos