Agradecimentos: This research was funded by the Triple Agenda Institutional Program of the Federal University of Latin American Integration (UNILA), grant number Call PRPPG 137/2018. S.S.d.S. was funded by The Brazilian National Council for Scientific and Technological Development (CNPq), grant number 303439/2018-1. S.C.d.A. was funded by the Araucária Foundation of Support to the Scientific and Technological Development of the State of Paraná (FAPPR). O.H.A.J. was funded by CNPq, grant numbers 307223/2017-5 and 407531/2018-1. E.C. was funded by INCTBio (FAPESP grant number 2014/50867-3 and CNPq grant number 465389/2014-7). D.T.S. was funded by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)-Finance Code 001 Ver menos

Abstract: Electrochemical biosensing devices are known for their simple operational procedures, low fabrication cost, and suitable real-time detection. Despite these advantages, they have shown some limitations in the immobilization of biochemicals. The development of alternative materials to overcome these drawbacks has attracted significant attention. Nanocellulose-based materials have revealed valuable features due to their capacity for the immobilization of biomolecules, structural flexibility, and biocompatibility. Bacterial nanocellulose (BNC) has gained a promising role as an alternative to antifouling surfaces. To widen its applicability as a biosensing device, BNC may form part of the supports for the immobilization of specific materials. The possibilities of modification methods and in situ and ex situ functionalization enable new BNC properties. With the new insights into nanoscale studies, we expect that many biosensors currently based on plastic, glass, or paper platforms will rely on renewable platforms, especially BNC ones. Moreover, substrates based on BNC seem to have paved the way for the development of sensing platforms with minimally invasive approaches, such as wearable devices, due to their mechanical flexibility and biocompatibility Ver menos