This research work aimed extraction and characterization of arrowroot starch. Besides, the effects of different concentrations of starch (2.59–5.41%, mass/mass) and concentrations of glycerol (9.95–24.08%, mass versus starch mass) on films properties were evaluated by a rotational central composite 22 experimental design. Arrowroot starch showed high amylose content (35%). Low values were found for the swelling power and solubility index. The X-ray diffraction showed “C” type crystalline structures, while thermogram showed Tg around of 118 and 120 °C. The thermogravimetric analysis showed that 40% of mass loss of starch occurred between 330 and 410 °C. The films were homogeneous, transparent and manageable. Starch and glycerol concentrations played a significant role in thickness and solubility in water of films, but was not significant for water vapor permeability and tensile strength. Therefore, arrowroot is a very promising starch source for application in films. Ver menos