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Type: Artigo de periódico
Title: Characterization Of Genetic Variability Of Kale Plants By Enzymatic Polymorphism And Rapd [caracterização Da Variabilidade Genética Em Couve-manteiga Utilizando Isoenzimas E Rapd]
Author: Sawazaki H.E.
Nagai H.
Sodek L.
Abstract: Genetic variability of kale plants (Brassica oleracea L. var. acephala D.C.) was studied by means of enzymatic polymorphism using polyacrilamide gel electrophoresis and a DNA polymorphism assay based on RAPD. Fifteen clones of kale var. acephala from IAC germplasm collection were studied. Leaf extracts were analysed for isozymes and RAPD markers using A and B kits of primers. Isozyme polymorphism was observed for phosphoglucose mutase (PGM), peroxidase (PRX) and esterase (EST) and was higher for PGM. Differences among clones were observed by isozymes and RAPD, however, the dendrograms obtained from both kinds of markers were dissimilar, suggesting that the isozymes provided less information than RAPD about the genome. The superior efficiency of the RAPD was due to its ability to process a larger number of samples, making details about genome more evident.
Rights: aberto
Identifier DOI: 
Date Issue: 1997
Appears in Collections:Unicamp - Artigos e Outros Documentos

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