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|Type:||Artigo de periódico|
|Title:||A New Amperometric Biosensor For Salicylate Based On Salicylate Hydroxylase Immobilized On Polipyrrole Film Doped With Hexacyanoferrate|
De Oliveira Neto G.
|Abstract:||An amperometric biosensor for salicylate detection was developed by immobilizing salicylate hydroxylase via glutaraldehyde onto a polypyrrole film doped with hexacyanoferrate, supported on a glassy carbon electrode surface. The sensor monitors the catechol produced in the enzymatic reaction on the film surface, at an applied potential of 150 mV vs. SCE. A [NADH]/[salicylate] ratio between 2 and 4 gave the best response. The biosensor presented the best performance in a solution with pH=7.4. The response time was about 40 s. A linear range of response was observed for salicylate concentrations between 1.0x10-5 and 1.0x10-4 mol l-1 and the equation adjusted for this curve was I=(-0.04±0.01)+(11.4±0.2)[salicylate] with a correlation coefficient of 0.999 for n=6. The biosensor retains its activity for at least 10 days despite daily use. The results obtained using the biosensor for salicylate determination, in three different samples of antithermic drugs, presented a good correlation with the standard colorimetric method.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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