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Type: Artigo de periódico
Title: A New Amperometric Biosensor For Salicylate Based On Salicylate Hydroxylase Immobilized On Polipyrrole Film Doped With Hexacyanoferrate
Author: Milagres B.G.
De Oliveira Neto G.
Kubota L.T.
Yamanaka H.
Abstract: An amperometric biosensor for salicylate detection was developed by immobilizing salicylate hydroxylase via glutaraldehyde onto a polypyrrole film doped with hexacyanoferrate, supported on a glassy carbon electrode surface. The sensor monitors the catechol produced in the enzymatic reaction on the film surface, at an applied potential of 150 mV vs. SCE. A [NADH]/[salicylate] ratio between 2 and 4 gave the best response. The biosensor presented the best performance in a solution with pH=7.4. The response time was about 40 s. A linear range of response was observed for salicylate concentrations between 1.0x10-5 and 1.0x10-4 mol l-1 and the equation adjusted for this curve was I=(-0.04±0.01)+(11.4±0.2)[salicylate] with a correlation coefficient of 0.999 for n=6. The biosensor retains its activity for at least 10 days despite daily use. The results obtained using the biosensor for salicylate determination, in three different samples of antithermic drugs, presented a good correlation with the standard colorimetric method.
Rights: fechado
Identifier DOI: 10.1016/S0003-2670(97)00105-0
Date Issue: 1997
Appears in Collections:Unicamp - Artigos e Outros Documentos

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