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Type: Artigo de periódico
Title: Identification Of A New Vascular Smooth Muscle Contracting Polypeptide In Phoneutria Nigriventer Spider Venom
Author: Bento A.C.
Novello J.C.
Marangoni S.
Antunes E.
Giglio J.R.
Oliveira B.
^de Nucci G.
Abstract: The fractionation of Phoneutria nigriventer spider venom by gel filtration (Sephadex G-10-120) followed by ion-exchange chromatography (microgranular CM-cellulose-52) resulted in sixteen fractions (C1 to CXVI) from which CVII+VIII, CIX and CX+XI caused dose-dependent and short-lived contractions of both arterial and venous rabbit vessels. Fraction CX+XI was further purified by a reverse phase HPLC, and a contractile polypeptide (PNV2) was isolated. The amino terminal sequence of PNV2 (LAKRADICQPGKTSQRACET) indicated that it represents a pure polypeptide consisting of a single chain. Furthermore, the amino acid analysis of PNV2 revealed the presence of four disulfide bridges, a high content in Lys (14%), Glx (11%), and the absence of His. The global amino acid composition showed that this polypeptide is composed of 102 residues (Trp not included) with a calculated molecular weight of 12,114. Whether this peptide is responsible for the vascular alterations observed in Phoneutria envenomation, such as lung edema and priapism, remains to be further investigated. © 1993.
Rights: fechado
Identifier DOI: 10.1016/0006-2952(93)90676-N
Date Issue: 1993
Appears in Collections:Unicamp - Artigos e Outros Documentos

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