Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/99586
Type: Artigo de periódico
Title: Calcium Homeostasis In Procyclic And Bloodstream Forms Of Trypanosoma Brucei: Lack Of Inositol 1,4,5-trisphosphate-sensitive Ca2+ Release
Author: Moreno S.N.J.
Docampo R.
Vercesi A.E.
Abstract: When Trypanosoma brucei procyclic trypomastigotes were permeabilized with digitonin in a reaction medium containing MgATP, succinate, and 3.5 μM free Ca2+, they lowered the medium Ca2+ concentration to the submicromolar level (0.05-0.1 μM), a range that correlates favorably with that detected in the intact cells with fura-2. The carbonyl cyanide p-trifluoromethoxyphenylhydrazone-insensitive Ca2+ uptake, certainly represented by the endoplasmic reticulum, was completely inhibited by 500 μM vanadate. When vanadate instead of carbonyl cyanide p-trifluoromethoxyphenylhydrazone was present, the Ca2+ set point was increased to 0.6-0.7 μM. The succinate dependence and carbonyl cyanide p-trifluoromethoxyphenylhydrazone sensitivity of the later Ca2+ uptake indicate that it may be exerted by the mitochondria. When bloodstream trypomastigotes were used, neither succinate nor α-glycerophosphate stimulated the mitochondrial Ca2+ uptake. The mitochondrial Ca2+ transport could be measured only in the presence of ATP and 500 μM vanadate to inhibit the endoplasmic reticulum uptake. Bloodstream trypomastigotes have a lower cytosolic Ca2+ concentration, as detected with fura-2 and a smaller extramitochondrial Ca2+ pool than procyclic trypomastigotes. Despite the presence of inositol phosphates, as determined by [3H]inositol incorporation, and the large extramitochondrial Ca2+ pool of procyclic trypomastigotes (61.7 nmol of Ca2+/mg of protein), no inositol 1,4,5-trisphosphate-sensitive Ca2+ release could be detected in these parasites.
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Rights: fechado
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Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-0026733656&partnerID=40&md5=a38cfe4a137ae9e8d95c284edd113e78
Date Issue: 1992
Appears in Collections:Unicamp - Artigos e Outros Documentos

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