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Type: Artigo de periódico
Title: Cloning And Characterization Of A Cdna Encoding A Sulfur-rich Coixin
Author: Leite A.
Yunes J.A.
Turcinelli S.R.
Arruda P.
Abstract: A full-length cDNA clone encoding a sulfur-rich Coix prolamin was isolated using a cDNA library constructed from polysomal mRNA prepared from immature Coix endosperm. The deduced amino acid sequence of the cDNA clone predicted a polypeptide of 194 residues, which shared 64% homology with the 17 kDa β-zein. The mature protein contains the familiar composition of the prolamins and an unusually high content of the sulfur-containing amino acids methionine (11.6%) and cysteine (5.2%). In vitro transcription followed by in vitro translation of the coding region of the pBCX17.9S clone gave rise to a polypeptide with an apparent molecular weight corresponding to the C4 α-coixin. Hydropathy analysis showed that C4 α-coixin is slightly more hydrophobic than β-zein. © 1992 Kluwer Academic Publishers.
Editor: Kluwer Academic Publishers
Citation: Plant Molecular Biology. Kluwer Academic Publishers, v. 18, n. 1, p. 171 - 174, 1992.
Rights: fechado
Identifier DOI: 10.1007/BF00018475
Date Issue: 1992
Appears in Collections:Unicamp - Artigos e Outros Documentos

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