Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/99411
Type: Artigo de periódico
Title: Cloning And Characterization Of A Cdna Encoding A Sulfur-rich Coixin
Author: Leite A.
Yunes J.A.
Turcinelli S.R.
Arruda P.
Abstract: A full-length cDNA clone encoding a sulfur-rich Coix prolamin was isolated using a cDNA library constructed from polysomal mRNA prepared from immature Coix endosperm. The deduced amino acid sequence of the cDNA clone predicted a polypeptide of 194 residues, which shared 64% homology with the 17 kDa β-zein. The mature protein contains the familiar composition of the prolamins and an unusually high content of the sulfur-containing amino acids methionine (11.6%) and cysteine (5.2%). In vitro transcription followed by in vitro translation of the coding region of the pBCX17.9S clone gave rise to a polypeptide with an apparent molecular weight corresponding to the C4 α-coixin. Hydropathy analysis showed that C4 α-coixin is slightly more hydrophobic than β-zein. © 1992 Kluwer Academic Publishers.
Editor: Kluwer Academic Publishers
Rights: fechado
Identifier DOI: 10.1007/BF00018475
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-0026442015&partnerID=40&md5=d390709a461cb9c3301fbb4c1d2e3b46
Date Issue: 1992
Appears in Collections:Unicamp - Artigos e Outros Documentos

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