Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/99384
Type: Artigo
Title: Changes In the nuclear phenotypes of Triatoma infestans Klug, induced by thermal shocks
Author: Dantas, Marly M.
Mello, Maria Luiza S.
Abstract: [No abstract available]
The nuclear phenotypes of Malpighian tubule epíthelial cells of 41h instar nymphs of Triatoma Infestans Klug were subjected to thermal shocks at o°c and 40°C for one and 12 hours and were analyzed just afterthe stress, and three and 30 days later.In addition to lhe phenotypes usually descríbed for control specímens, pyknotic, giant and vacuolízed nucleí, as well as nucleí exhíbíting an apparent unravellíng of chromocenter chromatin were detected in lhe test specímens, The decrease in number of cell nucleí, which occurred at shorter or longer intervals after lhe thermal shocks, were assumed to have been caused not on1y by degeneratíon of the cells bearing vacuolized and pyknotic nucleí, but also by nuclear fusion. It was assumed that lhe giant nucleí, which appeared predominantly in lhe specímens subjected to lhe hyperthermal shock, and lhe presence of hsp, played a role in lhe insect's unchanged survival under this specífic experimental condition. Although many of lhe nuclear characteristics found in lhe specímens subjected to thermal shock have a1so been observed with other stress agents, lhe heat shock was much more effective in elícitíng lhe apparent chrornocenter heterochromatín unravel1ing response in T. irfestans. Based on lhe types and frequencíes of nuclei observed after lhe hypothermal shock and 00 lhe drop in insect survival caused by this treatment, it seems that, if cryoprotectors are present in T. infestans, they are not effícíent enough to protect lhe ínsect from a relatívely prolonged hypothermal shock.
The nuclear phenotypes of Malpighian tubule epíthelial cells of 41h instar nymphs of Triatoma Infestans Klug were subjected to thermal shocks at o°c and 40°C for one and 12 hours and were analyzed just afterthe stress, and three and 30 days later.In addition to lhe phenotypes usually descríbed for control specímens, pyknotic, giant and vacuolízed nucleí, as well as nucleí exhíbíting an apparent unravellíng of chromocenter chromatin were detected in lhe test specímens, The decrease in number of cell nucleí, which occurred at shorter or longer intervals after lhe thermal shocks, were assumed to have been caused not on1y by degeneratíon of lhecells bearing vacuolized and pyknotic nucleí, but also by nuclear fusion. It was assumed that lhe giant nucleí, which appeared predominantly in lhe specímens subjected to lhe hyperthermal shock, and lhe presence of hsp, played a role in lhe insect's unchanged survival under this specífic experimental condition. Although many of lhe nuclear characteristics found in lhe specímens subjected to thermal shock have a1so been observed with other stress agents, lhe heat shock was much more effective in elícitíng lhe apparent chrornocenter heterochromatín unravel1ing response in T. irfestans. Based on lhe types and frequencíes of nuclei observed after lhe hypothermal shock and 00 lhe drop in insect survival caused by this treatment, it seems that, if cryoprotectors are present in T. infestans, they are not effícíent enough to protect lhe ínsect from a relatívely prolonged hypothermal shock.
metadata.dc.description.abstractalternative: Os fenótipos nucleares de células epiteliais dos túbulos de Malphigi de ninfas de 4• estadio de Triatoma infestans Klug submetidas a choques de temperatura a OOCe 40°C por 1e 12 horas foram investigados imediatamente após o estresse e 3 e 30 dias após o mesmo. Além dos fenótipos usualmente descritos para os espécimes controle, núcleos picnóticos, gigantes e vacuolizados, bem como núcleos com uma aparentedescompactação da cramatina do cromocentro foram detectados nos espécimes-teste. Admite-se que um decréscimo no número de núcleos a curto ou longo prazo após os choques de temperatura seja contribuído não só pela degeneração das células que apresentam núcleos vacuolizados e picnóticos, mas também por fusão nuclear. Supõe-se que os núcleos gigantes, que apareceram com predominância nos espécimes submetidos ao choque hípertérmíco e, ao mesmo tempo, a presença de hsp, desempenhem papel na manutenção da sobrevivência do inseto nesta condição experimental específica. Embora muitas das características nucleares encontradas nos espécimes submetidos aos choques de temperatura tenham sido também descritas após ação de outros agentes estressantes, o choque hípertérmíco foi o mais efetivo na promoção da aparente descompactação da heterocromatina do cromocentro. Com base nos tipos e frequências dos núcleos encontrados após choque hípertérmíco e queda na sobrevivência do inseto sob tal circunstância, considera-se que, se existem substâncias crioprotetoras em T. infestans, estas não seriam eficientes para proteger o inseto de um choque hipotérmico relativamente longo.
Subject: Túbulos de Malpighi
Triatoma infestans
Resposta ao choque térmico
Células epiteliais
Fenótipo
Células - Núcleo
Country: Brasil
Editor: Sociedade Brasileira de Genética
Citation: Revista Brasileira De Genetica. , v. 15, n. 3, p. 509 - 519, 1992.
Rights: fechado
Identifier DOI: 0
Address: https://www.gmb.org.br/brazilian-journal-of-genetics
Date Issue: 1992
Appears in Collections:IB - Artigos e Outros Documentos

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