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|Type:||Artigo de periódico|
|Title:||Detection Of Borrelia Burgdorferi In Biological Samples Using The Polymerase Chain Reaction Assay|
Van Elsen A.
|Abstract:||Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA regions of the Borrelia burgdorferi 49-kb linear plasmid. One set of primers identifies a 442-bp DNA fragment in the OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the OspB gene. The last set of primers. OspBpc3/pc4. outperformed the other pair in discriminating pathogenic North American or European isolates from related bacterial species, detected down to 4 spirochaetes, and was suitable for the identification of B. burgdorferi in biological samples, such as synovial and cerebrospinal fluids. © 1991.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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