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Type: Artigo de periódico
Title: Detection Of Borrelia Burgdorferi In Biological Samples Using The Polymerase Chain Reaction Assay
Author: Debue M.
Gautier P.
Hackel C.
Van Elsen A.
Herzog A.
Bigaignon G.
Bollen A.
Abstract: Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA regions of the Borrelia burgdorferi 49-kb linear plasmid. One set of primers identifies a 442-bp DNA fragment in the OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the OspB gene. The last set of primers. OspBpc3/pc4. outperformed the other pair in discriminating pathogenic North American or European isolates from related bacterial species, detected down to 4 spirochaetes, and was suitable for the identification of B. burgdorferi in biological samples, such as synovial and cerebrospinal fluids. © 1991.
Rights: fechado
Identifier DOI: 10.1016/0923-2508(91)90189-H
Date Issue: 1991
Appears in Collections:Unicamp - Artigos e Outros Documentos

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