Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/99188
Type: Artigo de periódico
Title: Damage To Rat Liver Mitochondria Promoted By δ-aminolevulinic Acid-generated Reactive Oxygen Species: Connections With Acute Intermittent Porphyria And Lead-poisoning
Author: Hermes-Lima M.
Valle V.G.R.
Vercesi A.E.
Bechara E.J.H.
Abstract: δ-Aminolevulinic acid is a heme precursor accumulated in acute intermittent porphyria and lead-poisoning, which supposedly triggers the typical clinical expression associated with these diseases. Considering that: (i) erythrocyte anti-oxidant enzymes are abnormally high in patients with both disorders and (ii) δ-aminolevulinic acid autoxidation generates reactive oxygen species, a possible contribution of reactive oxygen species in the pathophysiology of these disorders is explored here. Evidence is provided that δ-aminolevulinic acid (2-15 mM) induces damage to isolated rat liver mitochondria. Addition of δ-aminolevulinic acid disrupts the mitochondrial membrane potential, promotes Ca2+ release from the intramitochondrial matrix and releases the state-4 respiration, thus enhancing the permeability of the membrane to H+. The lesion was abolished by catalase, superoxide dismutase (both enzymes inhibit δ-aminolevulinic acid autoxidation) and ortho-phenanthroline, but not by mannitol; added H2O2 induces damage poorly. These results suggest the involvement of deleterious reactive oxygen species formed at particular mitochondrial sites from transition metal ions and δ-aminolevulinic acid-generated peroxide and/or superoxide species. These observations might be compatible with previous work showing hepatic mitochondrial damage in liver biopsy samples of acute intermittent porphyria patients. © 1991 Elsevier Science Publishers B.V. (Biomedical Division).
Editor: 
Rights: fechado
Identifier DOI: 10.1016/S0005-2728(05)80072-6
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-0026057381&partnerID=40&md5=9131152e8bbdc9c3643bc0af87673758
Date Issue: 1991
Appears in Collections:Unicamp - Artigos e Outros Documentos

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