Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/99124
Type: Artigo de periódico
Title: Determination Of Urea In Serum By Using Naturally Immobilized Urease In A Flow Injection Conductimetric System
Author: De Faria L.C.
Pasquini C.
De Oliveira Neto G.
Abstract: A flow injection method was developed, aimed at the determination of urea in human serum. The system makes use of the naturally immobilized urease present in Canavalia ensiformis DC (jack bean). A column is filled with small pieces of this bean, and the sample (50 μl) containing urea passes through it carried by a 1% NaCl solution. On leaving the column the stream is merged with an alkaline reagent (0.5 mol dm-3 NaOH; 0.5% disodium dihydrogen ethylenediaminetetraacetate). The ammonium ions, arising from the enzymatic reaction that occurs inside the column, are changed into the molecular form, which permeates a polytetrafluoroethylene membrane and is received in a de-ionized water acceptor stream. The ammonia ionizes causing an increase in the conductance, which is proportional to the urea content of the sample. About 40 samples can be processed in 1 h with negligible carry-over and with a relative standard deviation of 1% or less. The results are in agreement with those obtained by a standard spectrophotometric method.
Editor: 
Rights: aberto
Identifier DOI: 10.1039/AN9911600357
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-0026146861&partnerID=40&md5=e04b75792376ac0fe74906348d748c87
Date Issue: 1991
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
2-s2.0-0026146861.pdf490.75 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.