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|Type:||Artigo de periódico|
|Title:||Dna Strand Scission In E. Coli By Electronically Excited State Molecules Generated By Enzymatic Systems|
|Author:||De Toledo S.M.|
|Abstract:||E. coli containing pAT 153 plasmid undergoes strand scission when exposed to the indole-3-acetic acid/peroxidase/D2 system. Neither the initial components of this reaction nor the final stable products are responsible for this effect. Indole-3-aldehyde in its triplet state and singlet oxygen have been recently identified in this system. That singlet oxygen is one of the species acting on the plasmid in E. coli cells was suggested by protective effect of histidine and guanosine which are singlet oxygen quenchers. Similar effect on plasmid with malonaldehyde/peroxidase/O2 system was observed, which is an excellent singlet oxygen generator. This is the first report of a biological system where it is possible to detect a DNA scission in the intact cell by a bioenergized process. This presumably is related to spontaneous mutagenesis. © 1982.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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