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Type: Artigo de periódico
Title: Subcloning And Expression Of The α-amylase Gene From Bacillus Subtilis In Xanthomonas Campestris
Author: Stripecke R.
Rosato Y.B.
Astolfi-Filho S.
Abstract: A hybrid plasmid (pAP1) containing the α-amylase gene from Bacillus subtilis was constructed, using the pMFY40 plasmid as a cloning vector. The pAP1 plasmid was introduced into Xanthomonas campestris cells either by conjugation or transformation. The pAP1 plasmid proved to be stable under an antibiotic selection medium. The relative orientation of transcription of the α-amy gene in plasmid pMFY40 was deduced from single and double digestion with restriction enzymes. The expression of the amy gene was detected in non-amylolytic strains of X. campestris and Escherichia coli using an iodine staining assay in solid medium and measuring starch degradation and production of reducing sugars in liquid medium. © 1989 Springer-Verlag.
Editor: Springer-Verlag
Rights: fechado
Identifier DOI: 10.1007/BF00270786
Date Issue: 1989
Appears in Collections:Unicamp - Artigos e Outros Documentos

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