Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/98677
Type: Artigo de periódico
Title: Lysine-ketoglutarate Reductase Activity In Maize: Its Possible Role In Lysine Metabolism Of Developing Endosperm
Author: da Silva P.A.W.J.
Abstract: Lysine-ketoglutarate reductase has been isolated from maize (Zea mays) endosperm during development. The enzyme activity was dependent upon lysine, 2-oxoglutarate and NADPH as substrates. Several protein amino acids included in the assays at 10 mM concentration did not affect the enzyme activity. Lysine-ketoglutarate reductase activity increased sharply with the onset of endosperm development, reached a peak at 20 days after pollination and then declined towards seed maturity. The peak of activity coincided with the period of maximum rates of nitrogen and zein accumulation in the endosperm. Amino acid analyses of sap taken from the ear vascular system during ear development showed considerable amounts of lysine and other protein amino acids. Lysine found in the sap could account for more than twice the lysine required for protein synthesis in the endosperm. The results suggest that lysine-ketoglutarate reductase is associated with lysine breakdown in the endosperm during seed development. © 1983.
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Rights: fechado
Identifier DOI: 10.1016/S0031-9422(00)97673-8
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-0000082872&partnerID=40&md5=36eda03d4d6636604f1b30b90440d721
Date Issue: 1983
Appears in Collections:Unicamp - Artigos e Outros Documentos

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