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Type: Artigo de periódico
Title: Inhibition Of Ruthenium Red-induced Ca2+ Efflux From Liver Mitochondria By The Antibiotic X-537a
Author: de Silva L.P.
Bernardes C.F.
Vercesi A.E.
Abstract: It has been reported (Becker, G.L., Fiskum, G. and Lehninger, A.L. (1980) J.Biol.Chem.255, 9009-9012) that respiring rat liver mitochondria suspended in KCl medium containing ATP, Mg2+ and phosphate, maintain a steady state extramitochondrial free Ca2+ concentration of about 0.5 μM. The results reported here show that the addition of the antibiotic X-537A, at concentrations far below those required for ionophorous activity, caused a perturbation in this steady state, lowering the extramitochondrial free Ca2+ concentration by about 0.20 μM. This shift in steady state was clarified by a study of X-537A inhibition of the Ca2+ efflux induced by ruthenium red; a half-maximum effect was observed at approximately 25 nM X-537A. No effect on Ca2+ transport through the influx uniporter was observed. The possibility of a generalized stabilizing action of the antibiotic on the mitochondrial membrane seems to be ruled out by its effectiveness at very low concentrations. © 1984.
Rights: fechado
Identifier DOI: 10.1016/0006-291X(84)90919-7
Date Issue: 1984
Appears in Collections:Unicamp - Artigos e Outros Documentos

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