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|Type:||Artigo de periódico|
|Title:||Screening And Quantitation Of Ochratoxin A In Corn, Peanuts, Beans, Rice, And Cassava|
|Author:||Valente Soares L.M.|
|Abstract:||To answer the need for simple, economical, rapid methods for mycotoxins, a procedure for screening and quantitation of ochratoxin A was developed. A methanol-aqueous KCl extraction is used, followed by cleanup with clarifying agents and partition into chloroform. Part of the chloroform extract is used for screening and the other part for quantitation by thin layer chromatography (TLC). The screening procedure takes 40 min, using a silica gel/aluminum oxide minicolumn developed for this purpose. The limits of detection are 80 and 10 μg/kg, respectively, for minicolumn screening and TLC quantitation. Ammonium sulfate is efficient in cleaning samples of corn and cassava; cupric sulfate is better with peanuts, beans, and rice. Tests were conducted on triplicate spiked samples of yellow corn meal, raw peanuts, dried black beans, polished rice, and cassava flour at different levels (400, 200, 80, 40, and 10 μg/kg). Recoveries ranged from 86 to 160% and the coefficients of variation ranged from 0 to 26%.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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