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Type: Artigo de periódico
Title: Undesirable Feature Of Safranine As A Probe For Mitochondrial Membrane Potential
Author: Valle V.G.R.
Pereira-da-Silva L.
Vercesi A.E.
Abstract: This communication describes experiments showing that safranine, at the concentrations usually employed as a probe of mitochondrial membrane potential, causes significant undesirable side effects on Ca2+ transport by liver mitochondria. The major observations are: (i) safranine potentiates the spontaneous Ca2+ release from liver mitochondria induced by phosphate or acetoacetate. This is paralelled by potentiation of the release of state-4 respiration and of the rate of mitochondrial swelling, indicating a generalized effect of the dye on the mitochondrial membrane; (ii) the efflux of mitochondrial Ca2+ stimulated by hydroperoxide is irreversible in the presence of safranine even if membrane stabilizers such as Mg2+ and ATP are present. It is concluded that the use of safranine to monitor the changes in membrane potential during Ca2+ transport by mitochondria should be avoided or special care be taken. © 1986.
Rights: fechado
Identifier DOI: 10.1016/0006-291X(86)90961-7
Date Issue: 1986
Appears in Collections:Unicamp - Artigos e Outros Documentos

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