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|Type:||Artigo de periódico|
|Title:||Kinetic Properties Of Poly (2'-o-methylcytidylic Acid)-directed Reverse Transcriptase Reaction.|
|Abstract:||Poly (2'-O-methylcytidylic acid) is recognized as a template in reactions catalyzed by RNA-dependent DNA polymerases in the presence of Mn2+ as divalent cation. We report that kinetic data obtained for dGTP and template under optimal experimental conditions in the reaction catalyzed by reverse transcriptase showed some similarities between the poly (2'-O-methylcytidylic acid)/Mn2+ and polyribocytidylic acid/Mg2+ systems. The reaction was inhibited by the action of N-ethylmaleimide and novobiocin, and to a lesser extent by ethidium bromide and tetramethyl ethidium bromide.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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