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Type: Artigo de periódico
Title: Kinetic Properties Of Poly (2'-o-methylcytidylic Acid)-directed Reverse Transcriptase Reaction.
Author: Aoyama H.
Abstract: Poly (2'-O-methylcytidylic acid) is recognized as a template in reactions catalyzed by RNA-dependent DNA polymerases in the presence of Mn2+ as divalent cation. We report that kinetic data obtained for dGTP and template under optimal experimental conditions in the reaction catalyzed by reverse transcriptase showed some similarities between the poly (2'-O-methylcytidylic acid)/Mn2+ and polyribocytidylic acid/Mg2+ systems. The reaction was inhibited by the action of N-ethylmaleimide and novobiocin, and to a lesser extent by ethidium bromide and tetramethyl ethidium bromide.
Rights: aberto
Identifier DOI: 
Date Issue: 1988
Appears in Collections:Unicamp - Artigos e Outros Documentos

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