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Type: Artigo de periódico
Title: Specific Identification Of Bordetella Pertussis By The Polymerase Chain Reaction
Author: Houard S.
Hackel C.
Herzog A.
Bollen A.
Abstract: Oligonucleotide primers were used to amplify specific DNA regions of the Bordetella pertussis genome by the polymerase chain reaction. One pair of primers, PTp1/PTp2, identified a 191-bp DNA fragment located in the regulatory region of the pertussis toxin operon; a second pair of primers led to amplification of a 121-bp DNA piece located in an insertion-like element specific to B. pertussis. Both sets of primers were able to discriminate between the pathogen and related Bordetella species; they detected down to 6 bacteria and appeared suitable for routine detection of B. pertussis in clinical specimens. © 1989.
Rights: fechado
Identifier DOI: 10.1016/0923-2508(89)90069-7
Date Issue: 1989
Appears in Collections:Unicamp - Artigos e Outros Documentos

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