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|Type:||Artigo de periódico|
|Title:||Specific Identification Of Bordetella Pertussis By The Polymerase Chain Reaction|
|Abstract:||Oligonucleotide primers were used to amplify specific DNA regions of the Bordetella pertussis genome by the polymerase chain reaction. One pair of primers, PTp1/PTp2, identified a 191-bp DNA fragment located in the regulatory region of the pertussis toxin operon; a second pair of primers led to amplification of a 121-bp DNA piece located in an insertion-like element specific to B. pertussis. Both sets of primers were able to discriminate between the pathogen and related Bordetella species; they detected down to 6 bacteria and appeared suitable for routine detection of B. pertussis in clinical specimens. © 1989.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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