Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/97911
Type: Artigo de periódico
Title: Kinetics Of Oxygen And Carbon Monoxide Binding To The Hemoglobins Of The Water Snakes Liophis Miliaris And Helicops Modestus
Author: Focesi Jr. A.
Ogo S.H.
Bonaventura C.
Bonaventura J.
Abstract: 1. 1. Liophis miliaris and Helicops modestus are water snakes having different respiratory adaptiations to their specific habitats. L. miliaris is more active and spends more time on land than H. modestus. Knowledge of the equilibrium and kinetics of ligand binding to their hemoglobins leads to better understanding of molecular aspects of this adaptation. 2. 2. Both snakes contain several hemoglobin types in their blood. Studies on the kinetics of oxygen dissociation and carbon monoxide combination with these hemoglobins were performed by stopped-flow and flash-photolysis experiments at various pH values, both in the presence and absence of adenosine triphosphate. 3. 3. The oxygen dissociation kinetics of L. miliaris hemoglobins show a strong pH dependence and cooperative interactions between chains are indicated by autocatalytic time-courses at pH 7.0. In contrast, H. moledstus hemoglobins show nearly pH independent rate constants for oxygen dissociation and cooperative interactions between chains were not apparent. The hemoglobins of H. modestus show increased pH dependence in the presence of adenosine triphosphate. 4. 4. The carbon monoxide combination kinetics differ for the hemoglobins of L. miliaris and H. modestus in general agreement with the differences found in the kinetics and equilibria of oxygen binding. Both the kinetic and steady-state difference between these hemoglobins may be advantageous in light of the behavioral differences of these two water snakes. © 1980.
Editor: 
Rights: fechado
Identifier DOI: 10.1016/0305-0491(80)90414-9
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-0019207659&partnerID=40&md5=a6753329cd2f629e904230f5c353c609
Date Issue: 1980
Appears in Collections:Unicamp - Artigos e Outros Documentos

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