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Type: Artigo de periódico
Title: Determination Of Microgram Quantities Of Stevioside From Leaves Of Stevia Rebaudiana Bert. By Two-dimensional Thin Layer Chromatography
Author: Metivier J.
Viana A.M.
Abstract: A method is described for the determination of stevioside from leaves of Stevia rebaudiana Bert. Separation upon non-activated silica gel impregnated with boric acid in a two-dimensional chromatographic system followed by clution and colorimetric assay is reported. Recuperation from chromoplates is over 95%. The observed ratio of the optical density of glucose: stevioside matches that expected theoretically so that glucose may be employed as a standard where pure stevioside is not available.Effects of the plant growth retardant, ancymidol, on the growth and morphology of the shoot system of cucumber (Cucumis sativus L. ) were investigated. Ancymidol inhibited stem elongation, reducing both number and length of internodes. Reduction in leaf area, attributable to a reduction in both cell size and number, was accompanied by an increase in chlorophyll per unit area. The retardant decreased apical dominance and delayed anthesis. Gibberellic acid fully reversed ancymidol-induced inhibition of stem elongation, internode length and production, and leaf expansion. GA4/7 and ancymidol gave a synergistic promotion of stem elongation by increasing elongation of younger internodes and increasing internode production. Synergistic promotion of petiole elongation by this combination was also observed. Ancymidol, applied 7 d previously either to the shoot or root, severely reduced the level of gibberellin-like activity in bleeding sap collected from decapitated plants. © 1979 Oxford University Press.
Rights: fechado
Identifier DOI: 10.1093/jxb/30.4.805
Date Issue: 1979
Appears in Collections:Unicamp - Artigos e Outros Documentos

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