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Type: Artigo de periódico
Title: Expression Profile Of Apoptosis-related Genes In Childhood Adrenocortical Tumors: Low Level Of Expression Of Bcl2 And Tnf Genes Suggests A Poor Prognosis
Author: Lorea C.F.
Moreno D.A.
Borges K.S.
Martinelli Jr. C.E.
Antonini S.R.R.
De Castro M.
Tucci Jr. S.
Neder L.
Ramalho L.N.Z.
Cardinalli I.
Seidinger A.L.
Mastellaro M.J.
Yunes J.A.
Brandalise S.R.
Tone L.G.
Scrideli C.A.
Abstract: Background: Impaired apoptosis has been implicated in the development of childhood adrenocortical tumors (ACT), although the expression of apoptosis-related gene expression in such tumors has not been reported. Methods: The mRNA expression levels of the genes CASP3, CASP8, CASP9, FAS, TNF, NFKB, and BCL2 were analyzed by quantitative real-time PCR in consecutive tumor samples obtained at diagnosis from 60 children with a diagnosis of ACT and in 11 non-neoplastic adrenal samples. BCL2 and TNF protein expression was analyzed by immunohistochemistry. Results: A significant association was observed between tumor size ≥100 g and lower expression levels of the BCL2 (P=0.03) and TNF (P=0.05) genes; between stage IV and lower expression levels of CASP3 (P=0.008), CASP9 (P=0.02), BCL2 (P=0.002), TNF (P=0.05), and NFKB (P=0.03);Weiss score ≥3 and lower expression of TNF (P=0.01); unfavorable event and higher expression values of CASP9 (P=0.01) and lower values of TNF (P=0.02); and death and lower expression of BCL2 (P=0.04). Underexpression of TNF was associated with lower event-free survival in uni- and multivariate analyses (P<0.01). Similar results were observed when patients with Weiss score <3 were excluded. Conclusion: This study supports the participation of apoptosis-related genes in the biology and prognosis of childhood ACT and suggests the complex role of these genes in the pathogenesis of this tumor. © 2012 European Society of Endocrinology.
Rights: fechado
Identifier DOI: 10.1530/EJE-12-0183
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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