Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/97015
Type: Artigo
Title: Effect of bovine milk on Streptococcus mutans biofilm cariogenic properties and enamel and dentin demineralization
Author: Muñoz-Sandoval, C.
Muñoz-Cifuentes, M. J.
Giacaman, R. A.
Ccahuana-Vasquez, R. A.
Cury, J. A.
Abstract: Purpose: The purpose of this study was to evaluate the effect of whole bovine milk on Streptococcus mutans biofilms and on demineralization of enamel and root dentin. Methods: S mutans UA159 biofilms were cultured on enamel and dentin bovine slabs of known surface hardness (SH) and exposed (8x/day) to 10% sucrose (positive control), whole bovine milk, and 4.5% lactose. Biofilm acidogenicity was estimated by medium pH (2x/day). After 4 days for dentin and 5 days for enamel, biofilms were collected to obtain biomass, viable bacteria, total protein, and intra- and extracellular polysaccharides. Final SH was measured on the slabs. Demineralization was calculated as percentage of SH loss (%SHL). Differences were determined by analysis of variance and Tukey's test (P<.05). Results: Biofilms formed on both substrates under 10% sucrose showed higher acidogenicity, biomass, protein concentration, insoluble polysaccharides, and %SHL than those treated with milk or lactose (P<.05), which did not differ in any parameter (P>.05). Higher bacterial counts were obtained from biofilms treated with milk on enamel, but not on dentin. Dentin was significantly more demineralized by milk than enamel (P<.05). Conclusions: Although bovine milk appears to be less cariogenic than sucrose, this dairy product should not be considered caries-safe.
The purpose of this study was to evaluate the effect of whole bovine milk on Streptococcus mutans biofilms and on demineralization of enamel and root dentin. S mutans UA159 biofilms were cultured on enamel and dentin bovine slabs of known surface hardness (SH) and exposed (8x/day) to 10% sucrose (positive control), whole bovine milk, and 4.5% lactose. Biofilm acidogenicity was estimated by medium pH (2x/day). After 4 days for dentin and 5 days for enamel, biofilms were collected to obtain biomass, viable bacteria, total protein, and intra- and extracellular polysaccharides. Final SH was measured on the slabs. Demineralization was calculated as percentage of SH loss (%SHL). Differences were determined by analysis of variance and Tukey's test (P<.05). Biofilms formed on both substrates under 10% sucrose showed higher acidogenicity, biomass, protein concentration, insoluble polysaccharides, and %SHL than those treated with milk or lactose (P<.05), which did not differ in any parameter (P>.05). Higher bacterial counts were obtained from biofilms treated with milk on enamel, but not on dentin. Dentin was significantly more demineralized by milk than enamel (P<.05). Although bovine milk appears to be less cariogenic than sucrose, this dairy product should not be considered caries-safe
Subject: Cárie dentária
Lactose
Leite
Streptococcus mutans
Country: Estados Unidos
Editor: American Academy of Pediatric Dentistry
Citation: Pediatric Dentistry. , v. 34, n. 7, p. e197 - e201, 2012.
Rights: fechado
Address: http://www.ingentaconnect.com/contentone/aapd/pd/2012/00000034/00000007/art00007
Date Issue: 2012
Appears in Collections:FOP - Artigos e Outros Documentos

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