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Type: Artigo de periódico
Title: Development And Validation Of A Spectrophotometric Method For Determination Of Lutein Colorant Added To Nonfat Yogurt [desenvolvimento E Validação De Método Espectrofotométrico Para Determinação De Corante à Base De Luteína Adicionado Em Iogurte Desnatado]
Author: Xavier A.A.O.
Mercadante A.Z.
Domingos L.D.
Viotto W.H.
Abstract: A simple analytical method for extraction and quantification of lutein colorant added to yogurt was developed and validated. The method allowed complete extraction of carotenoids using tetrahydrofuran in vortex, followed by centrifugation, partition to diethyl ether/petroleum ether, and drying. The carotenoids dissolved in ethanol were quantified by UV-Vis spectrophotometry. This method showed linearity in the range tested (1.41-13.42 μg g -1), limits of detection and quantification of 0.42 and 1.28 μg g-1, respectively, low relative standard deviation (3.4%) and recovery ranging from 95 to 103%. The method proved reliable for quantification of lutein added to yogurt.
Rights: aberto
Identifier DOI: 10.1590/S0100-40422012001000028
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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