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|Type:||Artigo de periódico|
|Title:||Comparison Among Three Polymerase Chain Reaction Assays On Detection Of Dna From Leishmania In Biological Samples From Patients With American Cutaneous Leishmaniasis [comparação Entre Três Ensaios De Reação Em Cadeia Da Polimerase Na Detecção De Dna De Leishmania, Em Amostras Biológicas De Pacientes Com Leishmaniose Tegumentar Americana]|
|Author:||da Silva J.G.L.|
da Silva T.M.
e Silva P.M.F.
|Abstract:||Introduction: The study analyzed positivity of polymerase chain reaction (PCR) on detection of DNA from Leishmania in patients' samples. Methods: Extracted DNA was submitted to L150/L152, 13Y/13Z, and seminested PCR (snPCR). Results: Results were evidenced by bands of approximately 120, 720, and 670 bp for L150/L152, 13Y/13Z, and snPCR, respectively. L150/L152, 13Y/13Z, and snPCR positivity indexes were 76.9, 56.4, and 69.2 (p>0.05), respectively, for suspected and 93.7, 68.7, and 84.4 (p<0.05), respectively, for confirmed. Conclusions: Preliminary results showed that these assays, mainly L150/L152 and snPCR, can detect Leishmania DNA and carry potential on laboratory diagnosis of leishmaniasis.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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