Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/96639
Type: Artigo de periódico
Title: Comparison Among Three Polymerase Chain Reaction Assays On Detection Of Dna From Leishmania In Biological Samples From Patients With American Cutaneous Leishmaniasis [comparação Entre Três Ensaios De Reação Em Cadeia Da Polimerase Na Detecção De Dna De Leishmania, Em Amostras Biológicas De Pacientes Com Leishmaniose Tegumentar Americana]
Author: da Silva J.G.L.
da Silva T.M.
Peloso E.F.
Machado-Coelho G.L.L.
Mayrink W.
Ariosa M.C.F.
e Silva P.M.F.
Marques M.J.
Abstract: Introduction: The study analyzed positivity of polymerase chain reaction (PCR) on detection of DNA from Leishmania in patients' samples. Methods: Extracted DNA was submitted to L150/L152, 13Y/13Z, and seminested PCR (snPCR). Results: Results were evidenced by bands of approximately 120, 720, and 670 bp for L150/L152, 13Y/13Z, and snPCR, respectively. L150/L152, 13Y/13Z, and snPCR positivity indexes were 76.9, 56.4, and 69.2 (p>0.05), respectively, for suspected and 93.7, 68.7, and 84.4 (p<0.05), respectively, for confirmed. Conclusions: Preliminary results showed that these assays, mainly L150/L152 and snPCR, can detect Leishmania DNA and carry potential on laboratory diagnosis of leishmaniasis.
Editor: 
Rights: aberto
Identifier DOI: 10.1590/S0037-86822012000200023
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-84860365728&partnerID=40&md5=2a75ea35d9f2512a241d517493d6787a
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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