Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/96333
Type: Artigo de periódico
Title: Ca2+ Homeostasis And Mitochondrial Bioenergetics In Trypanosoma Cruzi.
Author: Vercesi A.E.
Abstract: Digitonin can be used to selectively permeabilize the plasma membrane of Trypanosoma cruzi without significantly affecting the functional integrity of mitochondria or the endoplasmic reticulum. This permits the study of functional properties of these subcellular organelles in situ, such as respiration, oxidative phosphorylation and Ca2+ transport. The ability of these cells to take up and hydrolyze Fura-2/AM, followed by the retention of the fluorescent Ca2+ indicator Fura-2, permits the determination of cytosolyc free Ca2+ concentrations in different T. cruzi stages.
Editor: 
Rights: aberto
Identifier DOI: 
Address: http://www.scopus.com/inward/record.url?eid=2-s2.0-0028379328&partnerID=40&md5=34782a5e290735b205d7714962640da8
Date Issue: 1994
Appears in Collections:Unicamp - Artigos e Outros Documentos

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