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|Type:||Artigo de evento|
|Title:||The Kinetics Of Chondroitin 4-sulfate Release From Stimulated Platelets And Its Relation To Thromboxane A2 Formation And Granule Secretion|
De Nucci G.
|Abstract:||In platelet rich plasma (PRP), chondroitin 4-sulfate release from platelets occurred after stimulation with ADP (5 μM), collagen (5-10 μg/ml), or adrenaline (10 μM). Release started within 60 s and maximum release (0.7-2.0 mg/l) was reached within 180 s. TXA2 formation and dense granule release reached a maximum within 90 s after stimulation. Using washed platelets (1.5 x 108 cells/ml), the platelet responses were faster. Release of chondroitin 4-sulfate and TXA2 started within 20-30 s after thrombin addition (100 mU/ml). Maximum release was reached within 60 s in both cases. Dense granule release started in the first 5 s of stimulation (34.6 ± 12.4%) reaching maximum secretion (74.4 ± 8.7%) within 60 s. Our results demonstrate that maximal chondroitin 4-sulfate release occurs after the dense granule release reaction in both PRP and washed platelets. This observation suggests that chondroitin 4-sulfate is unlikely to be stored in the dense granules but may be stored in the α-granules.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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