Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/961
Type: Artigo de periódico
Title: Vibrios dominate as culturable nitrogen-fixing bacteria of the Brazilian coral Mussismilia hispida
Author: CHIMETTO, Luciane A.
BROCCHI, Marcelo
THOMPSON, Cristiane C.
MARTINS, Roberta C. R.
RAMOS, Heloiza R.
THOMPSON, Fabiano L.
Abstract: Taxonomic characterization was performed on the putative N-2-fixing microbiota associated with the coral species Mussismilia hispida, and with its sympatric species Palythoa caribaeorum, P. variabilis, and Zoanthus solanderi, off the coast of Sao Sebastiao (Sao Paulo State, Brazil). The 95 isolates belonged to the Gammaproteobacteria according to the 16S rDNA gene sequences. In order to identify the isolates unambiguously, pyrH gene sequencing was carried out. The majority of the isolates (n = 76) fell within the Vibrio core group, with the highest gene sequence similarity being towards Vibrio harveyi and Vibrio alginolyticus. Nineteen representative isolates belonging to V. harveyi (n = 7), V. alginolyticus (n = 8), V. campbellii (n = 3), and V parahaemolyticus (n = 1) were capable of growing six successive times in nitrogen-free medium and some of them showed strong nitrogenase activity by means of the acetylene reduction assay (ARA). It was concluded that nitrogen fixation is a common phenotypic trait among Vibrio species of the core group. The fact that different Vibrio species can fix N, might explain why they are so abundant in the mucus of different coral species. (C) 2008 Published by Elsevier GmbH.
Subject: N-2-fixing bacteria
Vibrios
V. alginolyticus
V. harveyi
Coral
Mussismilia hispida
Palythoa caribaeorum
P. variabilis
Zoanthus solanderi
Country: Alemanha
Editor: ELSEVIER GMBH, URBAN & FISCHER VERLAG
Citation: SYSTEMATIC AND APPLIED MICROBIOLOGY, v.31, n.4, p.312-319, 2008
Rights: fechado
Identifier DOI: 10.1016/j.syapm.2008.06.001
Address: http://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000260357000007&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecord
http://dx.doi.org/10.1016/j.syapm.2008.06.001
Date Issue: 2008
Appears in Collections:IB - Artigos e Materiais de Revistas Científicas

Files in This Item:
File Description SizeFormat 
art_CHIMETTO_Vibrios_dominate_as_culturable_nitrogen-fixing_bacteria_of_2008.pdfpublished version223.06 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.