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|Type:||Artigo de periódico|
|Title:||Re-evaluating The Agnor Staining Response In Triton X-100-treated Liver Cells By Image Analysis|
|Author:||De Campos Vidal B.|
|Abstract:||The response of liver cell nuclei to a variant of the AgNOR method, which includes a treatment with Triton X-100 prior to staining assumed to improve the quality of the AgNOR-positive images, was compared to that of the usual method by image analysis procedures. The objective was to determine whether Triton X-100 removes proteins from the nuclei to the point of affecting the AgNOR response. Statistically significant differences were not found in comparisons of a nuclear area/AgNOR-stained area ratio nor in the number of AgNOR-positive dots/aggregates. It was thus assumed that the Triton X-100 treatment did not remove nuclear proteins likely to affect the AgNOR response, though it induced a cleaner background and, consequently, a clearer contrast between the stained areas and the background.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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